Human adipose tissue is a source of multipotent stem cells
Human adipose tissue is a source of multipotent stem cells
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DOI：
10.1091/mbc.e02-02-0105
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发表时间：
2002-12-01
影响因子：
3.3
通讯作者：
Hedrick, MH
中科院分区：
文献类型：
--
作者：
Zuk, PA;Zhu, M;Hedrick, MH
对成体干细胞进行的大部分工作集中在骨髓基质中发现的间充质干细胞（MSC）。脂肪组织，像骨髓一样，来源于胚胎间充质，并含有易于分离的基质。最近的初步研究已经确定了一个假定的干细胞群体内的脂肪基质室。这种细胞群，称为加工的脂肪抽吸物（PLA）细胞，可以从人脂肪抽吸物中分离，并且像MSC一样，向成骨、成脂、成肌和成软骨谱系分化。为了确认脂肪组织是否含有干细胞，使用几种分子和生物化学方法分析PLA群体和多个克隆分离物。PLA细胞表达多种CD标志物抗原，类似于在MSC上观察到的那些。PLA细胞和克隆的中胚层谱系诱导导致多个谱系特异性基因和蛋白质的表达。此外，生化分析也证实了谱系特异性活性。除了中胚层的能力，PLA细胞和克隆分化成推定的神经源性细胞，表现出神经元样形态和表达几种蛋白质与神经元表型一致。最后，PLA细胞表现出独特的特征，不同于在MSC中看到的那些，包括CD标记物谱和基因表达的差异。
Much of the work conducted on adult stem cells has focused on mesenchymal stem cells (MSCs) found within the bone marrow stroma. Adipose tissue, like bone marrow, is derived from the embryonic mesenchyme and contains a stroma that is easily isolated. Preliminary studies have recently identified a putative stem cell population within the adipose stromal compartment. This cell population, termed processed lipoaspirate (PLA) cells, can be isolated from human lipoaspirates and, like MSCs, differentiate toward the osteogenic, adipogenic, myogenic, and chondrogenic lineages. To confirm whether adipose tissue contains stem cells, the PLA population and multiple clonal isolates were analyzed using several molecular and biochemical approaches. PLA cells expressed multiple CD marker antigens similar to those observed on MSCs. Mesodermal lineage induction of PLA cells and clones resulted in the expression of multiple lineage-specific genes and proteins. Furthermore, biochemical analysis also confirmed lineage-specific activity. In addition to mesodermal capacity, PLA cells and clones differentiated into putative neurogenic cells, exhibiting a neuronal-like morphology and expressing several proteins consistent with the neuronal phenotype. Finally, PLA cells exhibited unique characteristics distinct from those seen in MSCs, including differences in CD marker profile and gene expression.