Detection of protein molecules by surface-enhanced Raman spectroscopy-based immunoassay using 2-5 nm gold nanoparticle lables
Detection of protein molecules by surface-enhanced Raman spectroscopy-based immunoassay using 2-5 nm gold nanoparticle lables
复制标题
DOI：
10.1002/jrs.1770
复制
发表时间：
2007-10-01
影响因子：
2.5
通讯作者：
Jana, Nikhil R.
中科院分区：
文献类型：
--
作者：
Manimaran, M.;Jana, Nikhil R.
在这里，我们报告了 2-5 nm 大小的金纳米颗粒标记的合成，用于基于表面增强拉曼光谱 (SERS) 的免疫分析，以检测蛋白质分子。 Au 纳米颗粒与异硫氰酸荧光素 (FITC) 和山羊抗 h-IgG（免疫球蛋白）结合，所得颗粒用于 h-IgG 的检测。市售的硝基纤维素条和银增强法用于基于 SERS 的免疫测定。 FITC 充当拉曼探针，该分子的振动指纹用于检测浓度范围为 1 至 100 ng/μl 的 h-IgG。我们的拉曼探针坚固耐用，尺寸小，具有高水溶性，在抗原抗体结合过程中具有最小的空间效应。版权所有 (c) 2007 John Wiley & Sons, Ltd.
Here we report the synthesis of 2-5 nm size gold nanoparticle labels for surface-enhanced Raman Spectroscopy (SERS) based immunoassay to detect protein molecules. The Au nanoparticles were conjugated with fluorescein isothiocyanate (FITC) and goat anti-h-IgG (immunoglobin) and the resultant particles were used for the detection of h-IgG. Commercially available nitrocellulose strip and silver enhancement method were used for SERS-based immunoassays. The FITC acts as a Raman probe, and vibrational fingerprint of this molecule was used for the detection of h-IgG in concentration ranging from 1 to 100 ng/mu l. Our Raman probe is robust and small in size and has high water solubility with minimum steric effect during antigen-antibody binding. Copyright (c) 2007 John Wiley & Sons, Ltd.