Excess fertilizer responsive miRNAs revealed in Linum usitatissimum L
Excess fertilizer responsive miRNAs revealed in Linum usitatissimum L
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DOI：
10.1016/j.biochi.2014.11.017
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发表时间：
2015-02-01
期刊：
影响因子：
3.9
通讯作者：
Kudryavtseva, Anna V.
中科院分区：
文献类型：
--
作者：
Melnikova, Nataliya V.;Dmitriev, Alexey A.;Kudryavtseva, Anna V.
有效的施肥对于提高作物产量和降低植物过量施用的风险是必要的。已知在土壤中不同营养浓度下，植物中microRNA（miRNAs）的表达水平发生变化。本研究的目的是鉴定和表征农业重要作物亚麻（Linum usitatissimum L.）在过量施肥下表达变化的miRNA。我们使用Illumina GAIIx对正常和过量肥料下生长的亚麻中的小RNA进行了测序。两个小RNA文库获得了超过1400万个原始读段。共鉴定出20个家族的84个保守miRNAs。根据高通量测序数据，揭示了几种亚麻miRNAs在过量施肥下的差异表达。对于6个miRNA家族（miR395、miR169、miR408、miR399、miR398和miR168），使用qPCR在扩展取样上评估表达水平改变。在过量肥料下，miR395显示出统计学显著的上调。已知miR395的靶基因参与硫酸盐吸收和同化。然而，根据我们的数据，miR395表达水平的改变不仅与过量的硫施用有关，而且与其他大量和微量营养素的冗余有关。此外，评估miRNA及其预测靶标的表达水平。首次在亚麻中发现miR399的表达与其预测的靶泛素结合酶E2基因的表达呈负相关。因此，我们认为miR399参与了L. usitatissimum。在我们的研究中揭示的表达变化有助于miRNA在亚麻对过量肥料的反应中发挥作用。(C)2014 Elsevier B.V.和法国生物化学与生物分子学会（SFBBM）。All rights reserved.
Effective fertilizer application is necessary to increase crop yields and reduce risk of plant overdosing. It is known that expression level of microRNAs (miRNAs) alters in plants under different nutrient concentrations in soil. The aim of our study was to identify and characterize miRNAs with expression alterations under excessive fertilizer in agriculturally important crop - flax (Linum usitatissimum L.). We have sequenced small RNAs in flax grown under normal and excessive fertilizer using Illumina GAIIx. Over 14 million raw reads was obtained for two small RNA libraries. 84 conserved miRNAs from 20 families were identified. Differential expression was revealed for several flax miRNAs under excessive fertilizer according to high-throughput sequencing data. For 6 miRNA families (miR395, miR169, miR408, miR399, miR398 and miR168) expression level alterations were evaluated on the extended sampling using qPCR. Statistically significant up-regulation was revealed for miR395 under excessive fertilizer. It is known that target genes of miR395 are involved in sulfate uptake and assimilation. However, according to our data alterations of the expression level of miR395 could be associated not only with excess sulfur application, but also with redundancy of other macro- and micronutrients. Furthermore expression level was evaluated for miRNAs and their predicted targets. The negative correlation between miR399 expression and expression of its predicted target ubiquitin-conjugating enzyme E2 gene was shown in flax for the first time. So we suggested miR399 involvement in phosphate regulation in L. usitatissimum. Revealed in our study expression alterations contribute to miRNA role in flax response to excessive fertilizer. (C) 2014 Elsevier B.V. and Societe francaise de biochimie et biologie Moleculaire (SFBBM). All rights reserved.