The non-enzymatic microbicidal activity of lysozymes
The non-enzymatic microbicidal activity of lysozymes
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DOI：
10.1016/s0014-5793(99)00405-6
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发表时间：
1999-04-23
期刊：
影响因子：
3.5
通讯作者：
Gieffers, W
中科院分区：
文献类型：
--
作者：
Düring, K;Porsch, P;Gieffers, W
T4溶菌酶被认为是通过其溶菌酶活性来破坏细菌的，然而，我们在这里证明了T4溶菌酶C-末端的两亲性螺旋延伸介导了其杀菌和抑真菌活性。在热变性的T4溶菌酶中，酶活性被完全消除，但出乎意料的是，抗微生物功能仍然保留。与T4溶菌酶的两亲性C-末端结构域对应的小合成肽显示杀微生物活性。它的膜干扰活性被直接证明为细菌、真菌和植物细胞，但不是在溶血测定中，用鸡蛋白色溶菌酶获得了可比较的结果，这为通过蛋白质工程优化溶菌酶作为各种应用中的抗微生物剂开辟了许多新的机会，（C）1999欧洲生物化学学会联合会。
T4 lysozyme was thought to destroy bacteria by its muramidase activity, However, we demonstrate here that amphipathic helix stretches in the C-terminus of T4 lysozyme mediate its bactericidal and fungistatic activities. In heat-denatured T4 lysozyme, the enzymatic activity is completely abolished but unexpectedly, the antimicrobial functions remain preserved. Small synthetic peptides corresponding to amphipathic C-terminal domains of T4 lysozyme show a microbicidal activity. Its membrane disturbing activity was directly demonstrated for bacterial, fungal and plant cells but not in a hemolysis assay, Comparable results were obtained with hen egg white lysozyme, This opens up many new opportunities for optimization of lysozymes as antimicrobial agents in various applications by protein engineering, (C) 1999 Federation of European Biochemical Societies.