The Drosophila Bruton’s Tyrosine Kinase (Btk) Homolog Is Required for Adult Survival and Male Genital Formation
The Drosophila Bruton’s Tyrosine Kinase (Btk) Homolog Is Required for Adult Survival and Male Genital Formation
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DOI：
10.1128/mcb.19.6.4405
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发表时间：
1999-06
影响因子：
5.3
通讯作者：
Kotaro Baba;Ayaha Takeshita;K. Majima;R. Ueda;S. Kondo;N. Juni;Daisuke Yamamoto
中科院分区：
文献类型：
--
作者：
Kotaro Baba;Ayaha Takeshita;K. Majima;R. Ueda;S. Kondo;N. Juni;Daisuke Yamamoto
摘要我们分离到一个果蝇fickleP（ficP）突变体，该突变体具有缩短的交配持续时间和缩短的成虫期寿命。ficP是发生在Btk基因中的一个内含子突变，该基因编码两种形式（1型和2型）的布鲁顿酪氨酸激酶（Btk）家族细胞质酪氨酸激酶，作为选择性外显子使用的结果。ficP突变阻止2型同种型的形成，但保留1型转录本的完整表达。普遍过度表达的野生型cDNA，通过使用热休克70启动子在后期幼虫或蛹阶段拯救的寿命和生殖缺陷的突变体，分别建立ficP表型和Btk基因突变之间的因果关系。拯救能力的阶段特异性表明Btk基因是雄性生殖器发育所必需的，也是成体生存所需的底物。
ABSTRACT We isolated a Drosophila fickleP(ficP) mutant with a shortened copulatory duration and reduced adult-stage life span. The reduced copulatory duration is ascribable to incomplete fusion of the left and right halves of the apodeme that holds the penis during copulation.ficP is an intronic mutation occurring in theBtk gene, a gene which encodes two forms (type 1 and type 2) of a Bruton’s tyrosine kinase (Btk) family cytoplasmic tyrosine kinase as a result of alternative exon usage. TheficP mutation prevents the formation of the type 2 isoform but leaves expression of the type 1 transcript intact. Ubiquitous overexpression of the wild-type cDNA by using a heat shock 70 promoter during the late larval or pupal stages rescued the life span and genital defects in the mutant, respectively, establishing the causal relationship between the ficP phenotypes and the Btk gene mutation. The stage specificity of the rescuing ability suggests that the Btk gene is required for the development of male genitalia and substrates required for adult survival.