Improved Method for Preparation of the Methyl Ester of Conjugated Linoleic Acid
Improved Method for Preparation of the Methyl Ester of Conjugated Linoleic Acid
复制标题
共轭亚油酸甲酯的改进制备方法
DOI：
10.5650/jos.50.237
复制
发表时间：
2001
影响因子：
1.5
通讯作者：
I. Ikeda
中科院分区：
文献类型：
--
作者：
T. Kamegai;M. Kasai;I. Ikeda
建立了以BF_3-甲醇溶液为甲基化试剂对共轭亚油酸进行定量分析的简便方法。在将25 mg共辄亚油酸添加到2 mL 14%BF 3-甲醇溶液中并在40°C下加热10 min后，似乎没有显著的CLA异构化为t，t-异构体或甲氧基伪产物的产生。将含有CLA的甘油三酯（25 mg）用1.5mL 0.5M NaOH-甲醇溶液在100 ℃下皂化7分钟，并向其中加入2 mL 14%BF 3-甲醇溶液，随后在40 ℃下加热10分钟。当以游离脂肪酸和甘油三酯形式分析时，使用十七烷酸作为内标测量的CLA回收率分别为99.7±0.3%（n=3）和99.7±0.4%（n=3）。因此，本发明的制备共轭亚油酸甲酯的改进方法似乎可用于食品中CLA的定量。
The authors established a simple method for conducting the quantitative analysis of conjugated linoleic acid using BF3-methanol solution as methylation reagent. Following the addition of 25 mg of conjugated linoleic acid to 2 mL 14% BF3-methanol solution and heating for 10min at 40°C, there appeared tp be no significant isomerization of CLA to t,t-isomers or production of methoxy artifacts. Triglyceride containing CLA (25 mg), was saponified with 1.5 mL 0.5 M NaOH-methanol solution for 7 min at 100°C and to which 2 mL 14% BF3-methanol solution were added followed by heating for 10 min at 40°C. CLA recovery, measured using heptadecanoic acid as internal standard, was 99.7±0.3% (n=3) and 99.7±0.4% (n=3), when analyzed in free fatty acid and triglyceride forms, respectively. The present improved method for preparing the methyl ester of conjugated linoleic acid would thus appear useful for the quantitation of CLA in foods.