STANDARDIZATION OF A RADIOCHEMICAL ASSAY OF CHOLINE ACETYLTRANSFERASE AND A STUDY OF THE ACTIVATION OF THE ENZYME IN RABBIT BRAIN
STANDARDIZATION OF A RADIOCHEMICAL ASSAY OF CHOLINE ACETYLTRANSFERASE AND A STUDY OF THE ACTIVATION OF THE ENZYME IN RABBIT BRAIN
复制标题
胆碱乙酰转移酶放射化学测定的标准化及兔脑酶激活的研究
DOI：
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发表时间：
1971
影响因子：
4.7
通讯作者：
B. Oderfeld‐Nowak
中科院分区：
文献类型：
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作者：
G. Bull;B. Oderfeld‐Nowak
摘要— 1 描述了胆碱乙酰转移酶（乙酰辅酶A：胆碱-O-乙酰转移酶 EC 2.3.1.6.[ChAc]）放射化学测定的标准化。该方法依赖于 [l-C14] 乙酰辅酶 A 作为底物的使用，并根据 MCCAMAN 和 HUNT (1965) 最初发表的程序进行了修改。 2 修改后的方法得出的结果与两种以生物测定为最终步骤的方法具有可比性，这两种方法之前给出了兔脑 ChAc 的最高记录值。 3 还研究了酶的提取和激活方法。结果表明，乙醚处理的蔗糖匀浆和丙酮干燥脑的半胱氨酸盐水提取物具有相当的酶活性；这两种方法都比未经处理的水匀浆或用 Nonex 501 或 Triton X-100 处理的蔗糖匀浆获得的值更高。
Abstract— 1 The standardization of a radiochemical assay of choline acetyltransferase (acetyl‐CoA: choline‐O‐acetyltransferase EC 2.3.1.6. [ChAc]) is described. The method depends upon the use of [l‐C14]acetyl‐CoA as substrate and has been modified from the procedure originally published by MCCAMAN and HUNT (1965). 2 The modified method gave results which were comparable with two methods depending on bioassay as the end step, which had previously given the highest recorded values for rabbit brain ChAc. 3 Methods of extraction and activation of the enzyme were also investigated. The results showed that ether‐treated sucrose homogenates and cysteine‐saline extracts of acetone‐dried brain have comparable enzyme activities; both procedures give higher values than are obtained with untreated water homogenates, or sucrose homogenates treated with Nonex 501 or Triton X‐100.