PixE promotes dark oligomerization of the BLUF photoreceptor PixD
PixE promotes dark oligomerization of the BLUF photoreceptor PixD
复制标题
PixE 促进 BLUF 感光器 PixD 的暗寡聚化
DOI：
--
复制
发表时间：
2008
影响因子：
11.1
通讯作者：
C. Bauer
中科院分区：
文献类型：
--
作者：
H. Yuan;C. Bauer
蓝细菌感知和移动（phototax）响应蓝光。在这项研究中，我们证明了PixD蓝光传感使用FAD（BLUF）光感受器，管理这种响应经历了光照时寡聚化状态的变化。在黑暗条件下，我们观察到PixD与另一种名为PixE的蛋白质形成了一种大分子量的复合物。化学计量分析，再加上沉降平衡和尺寸排阻色谱法，表明PixE驱动PixD二聚体聚集成一个稳定的PixD 10-PixE 5复合物在黑暗条件下。照射PixD中的黄素发色团使PixD 10-PixE 5复合物不稳定为PixE的单体和PixD的二聚体。PixD的晶体学结构，再加上PixD相互作用面之间的吉布斯自由能计算，有助于建立一个模型，其中光在关键的PixD接口环中诱导构象变化，导致PixD 10-PixE 5复合物的不稳定。
Cyanobacteria perceive and move (phototax) in response to blue light. In this study, we demonstrate that the PixD blue light-sensing using FAD (BLUF) photoreceptor that governs this response undergoes changes in oligomerization state upon illumination. Under dark conditions we observed that PixD forms a large molecular weight complex with another protein called PixE. Stoicheometric analyses, coupled with sedimentation equilibrium and size exclusion chromatography, demonstrates that PixE drives aggregation of PixD dimers into a stable PixD10—PixE5 complex under dark conditions. Illumination of a flavin chromophore in PixD destabilizes the PixD10–PixE5 complex into monomers of PixE and dimers of PixD. A crystallographic structure of PixD, coupled with Gibbs free energy calculation between interacting faces of PixD, lends to a model in which a light induces a conformational change in a critical PixD-interfacing loop that results in destabilization of the PixD10–PixE5 complex.
影响因子：
2.9
作者：
Yuan, Hua;Anderson, Spencer;Bauer, Carl
通讯作者：
Bauer, Carl