Protein/peptide transduction domains: potential to deliver large DNA molecules into cells.
Protein/peptide transduction domains: potential to deliver large DNA molecules into cells.
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发表时间：
2001-04
影响因子：
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通讯作者：
E. Snyder;S. Dowdy
中科院分区：
文献类型：
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作者：
E. Snyder;S. Dowdy
体内基因传递可以通过直接注射质粒DNA来实现。然而，低效率的细胞摄取和质粒DNA的核输入导致基因表达水平远低于使用病毒载体作为基因递送剂时所观察到的水平。最近的研究表明，转导肽，如HIV Tat蛋白，可以在体外和体内从细胞外环境直接携带大分子进入细胞质和细胞核。因此，tat介导的转导有可能增加质粒DNA在体内向细胞核的传递，从而增加基因表达。
In vivo gene delivery can be achieved by direct injection of plasmid DNA. However, inefficient cellular uptake and nuclear import of plasmid DNA result in much lower levels of gene expression than observed when viral vectors are used as gene delivery agents. Recent studies have shown that transducing peptides, such as the HIV Tat protein, can carry large biomolecules from the extracellular environment directly into the cytoplasm and the nucleus of cells, both in vitro and in vivo. Thus, TAT-mediated transduction has the potential to increase the delivery of plasmid DNA to the nuclei of cells in vivo and thereby increase gene expression.