How many conidia are required for wound infection of apple plants by Neonectria ditissima
How many conidia are required for wound infection of apple plants by Neonectria ditissima
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苹果树伤口被Neonectria ditissima感染需要多少分生孢子
DOI：
10.30843/nzpp.2016.69.5886
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发表时间：
2016
影响因子：
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通讯作者：
R. Scheper
中科院分区：
文献类型：
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作者：
M. Walter;S. Roy;B. M. Fisher;L. Mackle;N. T. Amponsah;T. Curnow;R. Campbell;P. Braun;A. Reineke;R. Scheper
采用温室盆栽、实验室离体盆栽和田间树木试验，研究了苹果品种“皇家嘎拉”和“Scilate”新鲜分支伤口上产生欧洲溃疡病所需的伤口大小和分生孢子数量。伤口类型为针刺伤、锉刀伤和修剪伤。孢子浓度从102至106分生孢子/ml，和两种接种方法（液滴和雾），使用。不同试验类型的疾病表达不同，可能是由于不同孵育条件对感染的诱导作用。总体而言，基于损伤类型、接种方法或孢子浓度>103分生孢子/ml，对病原体定殖和病变发展几乎没有影响。对于新鲜造成的伤口，例如修剪伤口或锉伤，在高度有利的条件下，在温室中只需3个分生孢子即可启动感染，在实验室中，离体枝条上只需12个分生孢子，在田间只需10至30个分生孢子。
A series of experiments, using potted plants in a glasshouse, detached laterals in the laboratory and trees in the field, were undertaken to study wound size and number of Neonectria ditissima conidia required to produce European canker infections on freshly-made branch wounds in the apple cultivars ‘Royal Gala’ and ‘Scilate’. The wound types were needle and pin injuries, rasp wounds and pruning cuts. Spore concentrations from 102 to 106 conidia/ml, and two inoculation methods (droplet and mist), were used. Disease expression varied for the different assay types, probably due to the conduciveness for infection of the different incubation conditions. Overall, there was little effect on pathogen colonisation and lesion development based on injury type, inoculation method or spore concentration >103 conidia/ml. For freshly-made wounds, such as pruning cuts or rasp wounds, only three conidia were required for infection initiation in the glasshouse under highly conducive conditions, 12 conidia in the laboratory on detached shoots, and 10 to 30 conidia in the field.