A novel inflammation-related lncRNAs prognostic signature identifies LINC00346 in promoting proliferation, migration, and immune infiltration of glioma.
A novel inflammation-related lncRNAs prognostic signature identifies LINC00346 in promoting proliferation, migration, and immune infiltration of glioma.
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一种新的炎症相关 lncRNA 预后特征鉴定出 LINC00346 可促进神经胶质瘤的增殖、迁移和免疫浸润
DOI：
10.3389/fimmu.2022.810572
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发表时间：
2022
影响因子：
7.3
通讯作者：
中科院分区：
文献类型：
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作者：
在这项研究中，通过单变量、多变量考克斯回归分析和LASSO分析，总共鉴定了13种具有高预后价值的炎症相关lncRNA。LINC 00346是13种lncRNA之一，与2型巨噬细胞活化和胶质瘤恶性程度呈正相关。荧光原位杂交（FISH）和免疫组化染色显示LINC 00346在高级别胶质瘤中高表达，而2型巨噬细胞关键转录因子STAT 3和表面标志物CD 204也同时高表达。LINC 00346高表达胶质瘤对抗PD-1和抗CTLA-4治疗更敏感。LINC 00346还与肿瘤增殖和肿瘤迁移相关，通过EdU、细胞集落、CCK 8形成和transwell测定验证。这些发现揭示了用于预测胶质瘤预后的新生物标志物，并概述了lncRNA炎症与胶质瘤之间的关系，以及胶质瘤的可能免疫检查点靶标。
In this study, a total of 13 inflammation-related lncRNAs with a high prognostic value were identified with univariate, multivariate Cox regression analysis, and LASSO analysis. LINC00346, which is one of the 13 lncRNAs identified, was positively associated with type 2 macrophage activation and the malignant degree of glioma. Fluorescence in situ hybridization (FISH) and immunohistochemical staining showed that LINC00346 was highly expressed in high-grade glioma, while type 2 macrophages key transcription factor STAT3 and surface marker CD204 were also highly expressed simultaneously. LINC00346 high-expression gliomas were more sensitive to the anti–PD-1 and anti-CTLA-4 therapy. LINC00346 was also associated with tumor proliferation and tumor migration validated by EdU, cell colony, formation CCK8, and transwell assays. These findings reveal novel biomarkers for predicting glioma prognosis and outline relationships between lncRNAs inflammation, and glioma, as well as possible immune checkpoint targets for glioma.
影响因子：
4.8
作者：
Hohman TJ;Koran ME;Thornton-Wells TA;Alzheimer's Neuroimaging Initiative
通讯作者：
Alzheimer's Neuroimaging Initiative