CNS neuronal focal adhesion kinase forms clusters that co‐localize with vinculin
CNS neuronal focal adhesion kinase forms clusters that co‐localize with vinculin
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CNS神经粘着斑激酶形成与纽蛋白共定位的簇
DOI：
--
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发表时间：
1996
影响因子：
4.2
通讯作者：
W. Klein
中科院分区：
文献类型：
--
作者：
Gerin R. Stevens;Chi Zhang;M. M. Berg;M. Lambert;K. Barber;I. Cantallops;A. Routtenberg;W. Klein
粘着斑激酶（FAK）是一种非受体蛋白酪氨酸激酶，似乎在非神经元细胞中的整合素介导的信号转导中发挥核心作用，在粘着斑接触处将细胞外基质连接到基于肌动蛋白的细胞骨架。生物化学分析揭示了FAK免疫反应性在神经元谱系细胞中的存在（Zhang et al.，1994）和CNS（Burgaya等，1995; Grant等，1995年）。在目前的工作中，我们已经研究了FAK的免疫分布在神经细胞培养和组织切片从大鼠中枢神经系统。B103 CNS神经母细胞瘤细胞培养物和原代培养的海马神经元均显示出丰富的FAK免疫反应阳性神经细胞体。免疫反应也延伸到神经突和生长锥。FAK分布的最显著特征是存在高FAK浓度的短点状簇。这些FAK簇保持在triton提取的细胞血影中，表明与细胞骨架相关。双标记共聚焦成像显示FAK簇与黏着斑蛋白簇一致，黏着斑蛋白是另一种与生长锥运动控制有关的肌动蛋白相关信号转导分子。来自海马切片的数据证实了FAK在成年神经元中的存在，其中FAK在体细胞-树突结构域中富集，并显示出非均匀分布。比较成人与胚胎脑的定量FAK免疫沉淀显示FAK发育下调7倍，FAK TyrP下调21倍。这些数据表明，神经元FAK可能参与相关的神经元形态发生和可塑性的信号转导复合物。© 1996 Wiley利斯公司
Focal adhesion kinase (FAK) is a non‐receptor protein tyrosine kinase that appears to play a central role in integrin‐mediated signal transduction in non‐neuronal cells, linking the extracellular matrix to the actin‐based cytoskeleton at focal adhesion contacts. Biochemical analysis has revealed the presence of FAK immunoreactivity in cells of neuronal lineage (Zhang et al., 1994) and in the CNS (Burgaya et al. 1995; Grant et al., 1995). In the current work, we have examined the immunodistribution of FAK in nerve cell cultures and tissue sections from the rat CNS. Cultures of B103 CNS neuroblastoma cells and primary cultures of hippocampal neurons both showed abundant FAK immunoreactivity in nerve cell bodies. Immunoreactivity also extended into neurites and growth cones. The most striking feature of FAK distribution was the presence of short, punctate clusters of high FAK concentration. These FAK clusters were maintained in triton‐extracted cell ghosts, indicating association with the cytoskeleton. Double‐label confocal imaging showed that clusters of FAK coincided with clusters of vinculin, another actin‐associated signal transduction molecule implicated in control of growth cone motility. Data from hippocampal sections verified the presence of FAK in adult neurons where it was enriched in somato‐dendritic domains and showed a non‐uniform distribution. Quantitative FAK immunoprecipitation to compare adult with embryonic brain showed a 7‐fold developmental down‐regulation of FAK and a 21‐fold down‐regulation of FAK TyrP. The data suggest that neuronal FAK may participate in signal transduction complexes relevant to neuronal morphogenesis and plasticity. © 1996 Wiley‐Liss, Inc.
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影响因子：
56.9
作者：
GRANT, SGN;ODELL, TJ;KANDEL, ER
通讯作者：
KANDEL, ER
DOI：
10.1073/pnas.88.19.8392
发表时间：
1991-10-01
影响因子：
11.1
作者：
KORNBERG, LJ;EARP, HS;JULIANO, RL
通讯作者：
JULIANO, RL
DOI：
10.1016/s0021-9258(18)35853-8
发表时间：
1992-11
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
L. Kornberg;H. Earp;J. Parsons;M. D. Schaller;Rudy L. Juliano
通讯作者：
L. Kornberg;H. Earp;J. Parsons;M. D. Schaller;Rudy L. Juliano
DOI：
10.1073/pnas.89.11.5192
发表时间：
1992-06-01
影响因子：
11.1
作者：
SCHALLER, MD;BORGMAN, CA;PARSONS, JT
通讯作者：
PARSONS, JT