A Feasibility Study of Nonlinear Spectroscopic Measurement of Magnetic Nanoparticles Targeted to Cancer Cells.
A Feasibility Study of Nonlinear Spectroscopic Measurement of Magnetic Nanoparticles Targeted to Cancer Cells.
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DOI：
10.1109/tbme.2016.2584241
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发表时间：
2017-05
期刊：
影响因子：
--
通讯作者：
Diamond SG
中科院分区：
文献类型：
--
作者：
Ficko BW;NDong C;Giacometti P;Griswold KE;Diamond SG
磁性纳米粒子（MNP）是一种用于癌症靶向诊断的新兴平台。 MNP 翻译所需的一个重要组成部分是检测和定量与肿瘤细胞结合的靶向 MNP。本研究探讨了多频非线性磁谱方法的可行性，该方法使用激励和拾波线圈，能够区分 KB 和 Igrov 人类癌细胞系 0.5 ml 样品中结合和未结合 MNP 的数量。该方法在 0 至 80 μg/ml 的五种浓度的 MNP 和每毫升 50 至 40 万个计数的五种浓度的细胞中进行了测试。应用于磁谱数据的线性模型能够同时测量结合和未结合的 MNP，模型拟合和实验室测定测量之间一致 (p<0.001)。在 0.5 ml 样品中，所提出的方法对结合和未结合 MNP 的可检测铁含量 < 2 μg。用于确定 KB 细胞中结合和未结合纳米颗粒数量的线性模型参数也用于测量 Igrov 细胞系中结合和未结合 MNP，反之亦然。磁性纳米颗粒的非线性光谱测量可能是研究肿瘤学中靶向 MNP 的有用方法。使用线性模型确定未知样品中结合和未结合的 MNP 的数量代表了将多频非线性光谱方法转化为 MNP 可以靶向癌细胞的体内应用的令人兴奋的机会。
Magnetic nanoparticles (MNPs) are an emerging platform for targeted diagnostics in cancer. An important component needed for translation of MNPs is the detection and quantification of targeted MNPs bound to tumor cells. This study explores the feasibility of a multi-frequency nonlinear magnetic spectroscopic method that uses excitation and pick-up coils and is capable of discriminating between quantities of bound and unbound MNPs in 0.5 ml samples of KB and Igrov human cancer cell lines. The method is tested over a range of five concentrations of MNPs from 0 to 80 μg /ml and five concentrations of cells from 50 to 400 thousand count per ml. A linear model applied to the magnetic spectroscopy data was able to simultaneously measure bound and unbound MNPs with agreement between the model-fit and lab assay measurements (p<0.001). The detectable iron of the presented method to bound and unbound MNPs was < 2 μg in a 0.5 ml sample. The linear model parameters used to determine the quantities of bound and unbound nanoparticles in KB cells were also used to measure the bound and unbound MNP in the Igrov cell line and vice versa. Nonlinear spectroscopic measurement of magnetic nanoparticles may be a useful method for studying targeted MNPs in oncology. Determining the quantity of bound and unbound MNP in an unknown sample using a linear model represents an exciting opportunity to translate multi-frequency nonlinear spectroscopy methods to in vivo applications where MNPs could be targeted to cancer cells.