Mapping chromosomal homologies between humans and two langurs (Semnopithecus francoisi and S-Phayrei) by chromosome painting
Mapping chromosomal homologies between humans and two langurs (Semnopithecus francoisi and S-Phayrei) by chromosome painting
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DOI：
10.1023/a:1009296227460
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发表时间：
1998-09-01
影响因子：
2.6
通讯作者：
Yang, FT
中科院分区：
文献类型：
--
作者：
Nie, WH;Liu, RQ;Yang, FT
用除叶猴外的所有人类染色体特异性DNA探针对两种叶猴（Semnopithecus francoisi, 2n=44， S. phayrei, 2n=44）进行染色体染色，确定了两种叶猴的染色体同源性。共有23个人类染色体特异性探针在单倍体叶猴基因组中检测到30个同源染色体片段。除了人类染色体1、2、6、16和19探针分别在两条非同源叶猴染色体上发出信号外，其他探针都是在一条染色体上杂交。结果表明，人类和这两种叶猴在染色体上具有高度的同源性。人类2号染色体探针绘制了叶猴14号和19号染色体的整个正染色区域。人类1号染色体探针对叶猴常染色体上的3个区域、4号染色体上的1个区域和5号染色体上的2个区域进行杂交。人类19号探针与叶猴4号染色体上的一个区域和5号染色体上的两个区域以相同的模式杂交，在那里它与人类1号染色体探针交替。人类6号和16号探针都与叶猴常染色体15号和18号上的一个区域杂交。只有两条叶猴染色体（12和21）分别被人类染色体（14和15,21和22）特异的探针标记。将这两种叶猴的人类绘画探针的杂交模式与其他东半球灵长类动物的数据进行比较表明，自人类和叶猴从共同祖先分化以来，可能发生了互惠和罗伯逊易位以及倒置。这一比较还表明，在核型上，亚洲的钶钽铁矿与非洲的钶钽铁矿之间的亲缘关系更为密切。
Chromosomal homologies were established between human and two Chinese langurs (Semnopithecus francoisi, 2n=44, and S. phayrei, 2n=44) by chromosome painting with chromosome-specific DNA probes of all human chromosomes except the Y. Both langur species showed identical hybridization patterns in addition to similar G-banding patterns. In total, 23 human chromosome-specific probes detected 30 homologous chromosome segments in a haploid langur genome. Except for human chromosomes 1, 2, 6, 16 and 19 probes, which each gave signals on two non-homologous langur chromosomes respectively, all other probes each hybridized to a single chromosome. The results indicate a high degree of conservation of chromosomal synteny between human and these two Chinese langurs. The human chromosome 2 probe painted the entire euchromatic regions of langur chromosomes 14 and 19. Human chromosome 1 probe hybridized to three regions on langur autosomes, one region on langur chromosome 4 and two regions on langur chromosome 5. Human 19 probe hybridized on the same pattern to one region on chromosome 4 and to two regions on langur chromosome 5, where it alternated with the human chromosome 1 probe. Human 6 and 16 probes both hybridized to one region on each of the two langur autosomes 15 and 18. Only two langur chromosomes (12 and 21) were each labelled by probes specific for two whole human chromosomes (14 and 15 and 21 and 22 respectively). Comparison of the hybridization patterns of human painting probes on these two langurs with the data on other Old World primates suggests that reciprocal and Robertsonian translocations as will as inversions could have occurred since the divergance of human and the langurs from a common ancestor. This comparison also indicates that Asian colobines are karyotypically more closely related to each other that to African colobines.