Enhanced enzymatic hydrolysis of corncob by ultrasound-assisted soaking in aqueous ammonia pretreatment
Enhanced enzymatic hydrolysis of corncob by ultrasound-assisted soaking in aqueous ammonia pretreatment
复制标题
超声辅助氨水浸泡预处理强化酶解玉米芯
DOI：
10.1007/s13205-018-1186-2
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发表时间：
2018-03-08
期刊：
影响因子：
2.8
通讯作者：
He, Zhimin
中科院分区：
文献类型：
--
作者：
Du, Ruoyu;Su, Rongxin;He, Zhimin
以15wt%氨水为原料，在低温（60 ℃左右）、短时（< 12 min）条件下，研究了超声辅助氨水浸泡（USAA）对玉米芯酶解的强化作用。对影响纤维素回收率、脱木素率和糖回收率的能量密度（2.93-17.07 W/mL）和超声时间（0.34-11.66 min）进行了研究和优化。在最佳条件下（能量密度10 W/mL，超声时间11.66 min）测定的最大纤维素回收率，脱木素和糖回收率分别为83.8%，84.7%和77.6%。用USAA预处理的玉米芯与用氨水浸泡（SAA）预处理的相比，半纤维素含量较低（28.9%对31.8%），结晶度指数值略低（42.7%对43.7%），表面空腔直径较大（> 36 μ m对< 20 μ m）。实验证明，USAA预处理是一种可靠、有效的玉米芯预处理方法。
Ultrasound-assisted soaking in aqueous ammonia (USAA) pretreatment with 15 wt% aqueous ammonia under low temperature (similar to 60 degrees C) and short-time (< 12 min) low-frequency (20 kHz, 60-650 W) ultrasound has been investigated for enhancement of enzymatic hydrolysis of corncob. Operational parameters of energy density (2.93-17.07 W/mL) and sonication time (0.34-11.66 min) that affect cellulose recovery, delignification, and sugar recovery yield were studied and optimized. The maximum cellulose recovery, delignification and sugar recovery yield determined at the optimum conditions (energy density 10 W/mL, sonication time 11.66 min) were 83.8, 84.7, and 77.6%, respectively. The corncob pretreated using USAA has a lower hemicellulose content (28.9% vs 31.8%), a slightly lower crystallinity index value (42.7% vs 43.7%), and a larger surface cavity diameter (> 36 mu m vs < 20 mu m) than that pretreated using soaking in aqueous ammonia (SAA) pretreatment. The USAA pretreatment was proved to be a reliable and effective method for corncob pretreatment.