The effects of surface properties on Escherichia coli adhesion are modulated by shear stress
The effects of surface properties on Escherichia coli adhesion are modulated by shear stress
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DOI：
10.1016/j.colsurfb.2014.08.016
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发表时间：
2014-11-01
影响因子：
5.8
通讯作者：
Mergulhao, F. J.
中科院分区：
文献类型：
--
作者：
Moreira, J. M. R.;Araujo, J. D. P.;Mergulhao, F. J.
在平行板流动室中监测大肠杆菌在不同流速（1 - 10 ml s（-1））下对玻璃和聚二甲基硅氧烷（PDMS）的粘附，以了解表面性质和流体动力学条件对粘附的影响。计算流体动力学被用来评估该流动室在相关生物医学系统的流体动力学模拟中的适用性。获得了0.005和0.07 Pa之间的壁面剪切应力，这些与循环系统、生殖系统和泌尿系统中的壁面剪切应力相似。结果表明，E.通过剪切应力调节对疏水PDMS和亲水玻璃表面的科尔粘附，其中表面性质在测试的较低和最高流速下具有较强的影响，而在中间流速下具有可忽略的影响。这些发现表明，当选择昂贵的材料或涂层来生产生物医学器械时，这种选择应考虑到在使用这些器械期间将发生的生理流体动力学条件。
The adhesion of Escherichia coli to glass and polydimethylsiloxane (PDMS) at different flow rates (between 1 and 10 ml s(-1)) was monitored in a parallel plate flow chamber in order to understand the effect of surface properties and hydrodynamic conditions on adhesion. Computational fluid dynamics was used to assess the applicability of this flow chamber in the simulation of the hydrodynamics of relevant biomedical systems. Wall shear stresses between 0.005 and 0.07 Pa were obtained and these are similar to those found in the circulatory, reproductive and urinary systems. Results demonstrate that E. coll adhesion to hydrophobic PDMS and hydrophilic glass surfaces is modulated by shear stress with surface properties having a stronger effect at the lower and highest flow rates tested and with negligible effects at intermediate flow rates. These findings suggest that when expensive materials or coatings are selected to produce biomedical devices, this choice should take into account the physiological hydrodynamic conditions that will occur during the utilization of those devices.