Metatranscriptome analysis reveals environmental and diel regulation of a Heterosigma akashiwo (raphidophyceae) bloom
Metatranscriptome analysis reveals environmental and diel regulation of a Heterosigma akashiwo (raphidophyceae) bloom
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宏转录组分析揭示了赤潮异藻（Raphidophyceae）水华的环境和昼夜调节
DOI：
10.1111/1462-2920.14045
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发表时间：
2018-03-01
影响因子：
5.1
通讯作者：
Lin, Senjie
中科院分区：
文献类型：
--
作者：
Ji, Nanjing;Lin, Lingxiao;Lin, Senjie
尽管有害藻华（HAB）物种的生理学的许多实验室研究，这些藻类在自然水华的生理学研究不足。本文利用18S rDNA和元转录组测序技术对2014年东海赤潮异弯藻（Heterosigma akashiwo）水华进行了研究。18S rDNA分析表明，浮游植物群落由盛前期的高多样性向盛后期的高多样性转变。在开花期间，赤穗占优势。环境中溶解无机磷的急剧下降和磷酸盐和溶解有机磷（DOP）吸收基因的强烈上调，包括很少记录的（ppGpp）酶，在H。从开花前到开花，赤藻对磷的快速吸收和对DOP的有效利用可能是H.明志沃·布鲁姆。此外，观察到的混合营养相关基因的表达上调，表明潜在的贡献，混合营养的开花。开花期间碳酸酐酶基因的上调也暗示了光合固碳的加速。值得注意的是，我们还观察到许多基因的表达从早上到下午有很强的变化。我们的研究结果提供了对代谢过程的见解，可能对H。赤潮的形成，并建议需要考虑在实地研究中对这种赤潮的采样时间。
Despite numerous laboratory studies on physiologies of harmful algal bloom (HAB) species, physiologies of these algae during a natural bloom are understudied. Here, we investigated a bloom of the raphidophyte Heterosigma akashiwo in the East China Sea in 2014 using metabarcode (18S rDNA) and metatranscriptome sequencing. Based on 18S rDNA analyses, the phytoplankton community shifted from high diversity in the pre-bloom stage to H. akashiwo predominance during the bloom. A sharp decrease in ambient dissolved inorganic phosphate and strong up-regulation of phosphate and dissolved organic phosphorus (DOP) uptake genes, including the rarely documented (ppGpp)ase, in H. akashiwo from pre-bloom to bloom was indicative of rapid phosphorus uptake and efficient utilization of DOP that might be a driver of the H. akashiwo bloom. Furthermore, observed up-regulated expression of mixotrophy-related genes suggests potential contribution of mixotrophy to the bloom. Accelerating photosynthetic carbon fixation was also implied by the up-regulation of carbonic anhydrase genes during the bloom. Notably, we also observed a strong morning-to-afternoon shift in the expression of many genes. Our findings provide insights into metabolic processes likely important for H. akashiwo bloom formation, and suggest the need to consider timing of sampling in field studies on this alga.