PLATELET STORAGE LESIONS IN 2ND-GENERATION CONTAINERS - CORRELATION WITH INVIVO BEHAVIOR WITH STORAGE UP TO 14 DAYS
PLATELET STORAGE LESIONS IN 2ND-GENERATION CONTAINERS - CORRELATION WITH INVIVO BEHAVIOR WITH STORAGE UP TO 14 DAYS
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DOI：
10.1111/j.1423-0410.1990.tb02106.x
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发表时间：
1990-01-01
期刊：
影响因子：
2.7
通讯作者：
WHITLEY, P
中科院分区：
文献类型：
--
作者：
HOLME, S;HEATON, WAL;WHITLEY, P
本文系统地研究了59份血小板浓缩物（PC）在合成培养基或CPDA-1血浆中储存长达14天的体内行为与体外特性之间的关系。进行了25项配对研究（1项研究未完成），比较了储存5天（n = 5）、7天（n = 10）、10天（n = 5）和14天（n = 5）的悬浮在合成培养基或CPDA-1血浆中的血小板。此外，对10例对照受试者进行了新鲜制备的PC（6-24 h）CPDA-1血浆给药。回收率和存活率估计值均随储存时间的增加而降低，与使用的储存介质无关。在这两种介质中，随着储存时间的延长，血小板存活曲线不仅变得更短，而且越来越指数，这表明体外储存对循环中存在的血小板造成了进行性损伤。悬浮在合成培养基中的血小板的存活曲线保持更线性，表明随机损伤比储存更少。储存PC的平均群体寿命（MPL）由存活曲线下的面积除以新鲜PC的平均回收率（55%）确定。在血浆中储存14天后，MPL从4.5天（新鲜PC）降至0.4天，估计储存7.2天时降低50%（t1/2）。在合成培养基中储存的PC的MPL t1/2估计为8.8天。随着储存时间的延长，体内活力的降低与能量依赖性体外参数的丧失有关，如低渗休克反应、ADP和ATP水平的形状变化以及乳酸水平的增加。虽然储存期的长度被证明是主要因素，负责在本研究中观察到的MPL的变异性，多元线性回归分析也表明，血小板盘状形状，测量的形状变化，和乳酸的生产是独立的体外参数与显着的可预测性的体内活力。结合回归方程中的储存天数，发现这些参数可以“解释”本研究中MPL变异性的78%。
The relationship between in vivo behavior and in vitro characteristics of 59 platelet concentrates (PC) stored for up to 14 days in a synthetic medium or in CPDA-1 plasma was systematically investigated. 25 paired studies (1 study was incomplete) were performed comparing platelets suspended either in the synthetic medium or CPDA-1 plasma with 5 days (n = 5); 7 days (n = 10); 10 days (n = 5); and 14 days (n = 5) of storage. In addition, 10 control subjects were performed with freshly prepared PC (6-24 h) in CPDA-1 plasma. Both percent recovery and survival estimations showed decreases with increasing storage duration, irrespective of storage medium used. In both media, with prolonged storage, the platelet survival curves not only became shorter, but also increasingly exponential, suggesting that in vitro storage caused progressive damage to the platelets present in circulation. Survival curves of platelets suspended in synthetic medium remained more linear, indicative of less random damage than storage. Mean population lifespan (MPL) of the stored PC was determined by the area below the survival curve divided by the mean percent recovery for the fresh PC, which was 55%. MPL decreased from 4.5 days (fresh PC) to 0.4 days after 14 days of storage in plasma, with a 50% reduction (t1/2) estimated at 7.2 days of storage. MPL t1/2 for PC stored in the synthetic medium was estimated to be 8.8 days. The decrease in in vivo viability with prolonged storage was paralleled with loss of energy-dependent in vitro parameters such as hypotonic shock response, shape change with ADP, and ATP levels, and with increased lactate levels. Although the length of the storage period was shown to be the major factor responsible for the variability in MPL observed in this study, multiple linear-regression analysis showed also that platelet discoid shape, as measured by the shape change, and lactate production were independent in vitro parameters with significant predictability of the in vivo viability. Combined with days of storage in the regression equation, these parameters were found to ''explain'' 78% of the variability of the MPL found in this study.