Polyurethane conjugating TGF-beta on surface impacts local inflammation and endoplasmic reticulum stress in skeletal muscle
Polyurethane conjugating TGF-beta on surface impacts local inflammation and endoplasmic reticulum stress in skeletal muscle
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表面结合 TGF-β 的聚氨酯影响骨骼肌局部炎症和内质网应激
DOI：
10.1002/jbm.a.35999
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发表时间：
2017
影响因子：
4.9
通讯作者：
Liao Hua
中科院分区：
文献类型：
--
作者：
Shi D;an;Xiao Jiangwei;Gu Ruicai;Wu Gang;Liao Hua
合成的短肽聚合物将通过在聚合物上富集生物活性分子或释放这些预先缀合在材料上的分子，为组织再生和修复提供关键功能。我们已经开发了一种可降解的聚氨酯（PU）轴承HSNGLPL肽，它具有亲和结合转化生长因子β（TGF-β）的能力。为了深入了解TGF-β在聚氨酯聚合物中的空间释放及其局部生物活性，采用石英晶体微天平（QCM）和Elisa试验验证了TGF-β在体外和体内的结合能力。将具有或不具有TGF-β预缀合的PU聚合物植入C57 BL/6小鼠的胃肌（GN）中，以解决TGF-β从聚合物的释放及其体内生物调节功能。QCM结果表明，携带HSNGLPL肽的PU在体外具有与TGF-β的亲和结合能力。肌内植入实验进一步支持了TGF-β对体内PU聚合物的富集效率。通过对植入物引发的肌内炎症浸润、肌纤维再生、肌肉纤维化程度、内质网应激（ER应激）激活等检测数据，证明了TGF-β可以从PU聚合物中释放出来，并对材料诱导的炎症发挥调节作用。因此，我们目前的结果表明，通过在植入前在材料上预载生物活性分子来改善PU聚合物的体内生物相容性是可行的。© 2017 Wiley Periodicals，Inc. J Biomed Mater Res Part A：105A：1156-1165，2017.
The synthesized short peptide‐polymers would provide key functions for tissue regeneration and repair, through enriching bioactive molecules on polymers or releasing these molecules pre‐conjugated on the materials. We have developed a degradable polyurethane (PU) bearing HSNGLPL peptide, which has affinity binding ability to transforming growth factor‐betas (TGF‐β). For deeply understanding spatial release of TGF‐β from the PU polymers and its localized bioactivity, quartz crystal microbalance (QCM) and Elisa test were used to verify TGF‐β binding capacitiesin vitroandin vivo. The PU polymers, with or without pre‐conjugating of TGF‐β, were implanted into gastronomies muscle (GN) of C57BL/6 mice, for addressing TGF‐β release from the polymers and its bio‐regulating functionin vivo. QCM result shows that PU bearing HSNGLPL peptide has affinity binding ability to TGF‐βin vitro. Intramuscular implanting experiment further supports the enrichment efficiency of TGF‐β on PU polymersin vivo. The detecting data involving intramuscular inflammatory infiltration triggered by the implants, myofiber regeneration, muscular fibrosis degree, and activation of endoplasmic reticulum stress (ER stress), evidence TGF‐β can be released from PU polymers, and exerts regulating effects on the material‐induced inflammation. Thus, our present results suggest it is feasible to improve biocompatibility of PU polymersin vivo, by pre‐bearing bioactive molecules on materials before the implanting. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1156–1165, 2017.