Intestinal cytodifferentiation in vitro of chick stomach endoderm induced by the duodenal mesenchyme.
Intestinal cytodifferentiation in vitro of chick stomach endoderm induced by the duodenal mesenchyme.
复制标题
十二指肠间充质诱导鸡胃内胚层体外肠细胞分化。
DOI：
--
复制
发表时间：
1984
期刊：
影响因子：
--
通讯作者：
T. Mizuno
中科院分区：
文献类型：
--
作者：
A. Ishizuya‐Oka;T. Mizuno
用电镜和免疫荧光技术研究了十二指肠间充质对鸡胚胃内胚层细胞分化的诱导作用。形态未分化的内胚层的胃的4天的胚胎只能分化成一个粘液分泌上皮时，培养在没有间充质。而与6日龄的十二指肠间充质复合培养时，4日龄的胃内胚层细胞大多分化为肠吸收细胞和杯状细胞，但未分化为胃型粘液分泌细胞。相反，当4天的胃内胚层与胚胎消化器官而不是肠的间充质重组培养时，没有胃内胚层细胞分化为肠上皮细胞。随着发育，胃内胚层的肠细胞分化能力迅速下降，但仍保持到相对较晚的阶段，在砂囊区。目前的研究表明，十二指肠间充质可以诱导胃内胚层，这已经获得了自我分化为胃型上皮的潜力，细胞分化为肠上皮。
The inductive action of duodenal mesenchyme on the cytodifferentiation of stomach endoderm in chick embryos was investigated in vitro with electron microscopy and immunofluorescence. Morphologically undifferentiated endoderm of the stomach of a 4-day embryo could differentiate only into a mucous secretory epithelium when cultured in the absence of mesenchyme. However, when cultivated in recombination with 6-day duodenal mesenchyme, most cells of 4-day stomach endoderm differentiated into intestinal absorptive cells possessing striated border and sucrase, and goblet cells, but not into stomach-type mucous secretory cells. In contrast, when 4-day stomach endoderm was cultured recombined with mesenchyme of embryonic digestive organs other than intestine, none of the stomach endoderm cells differentiated into intestinal epithelial cells. The competence of stomach endoderm for intestinal cytodifferentiation decreased rapidly with development, but remained until relatively later stages in the gizzard region. The present investigation demonstrates that duodenal mesenchyme can induce stomach endoderm, which has acquired the potency for self-differentiation into stomach-type epithelium, to cytodifferentiate into intestinal epithelium.