Shugoshin forms a specialized chromatin domain at subtelomeres that regulates transcription and replication timing.
Shugoshin forms a specialized chromatin domain at subtelomeres that regulates transcription and replication timing.
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DOI：
10.1038/ncomms10393
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发表时间：
2016-01-25
影响因子：
16.6
通讯作者：
Kanoh J
中科院分区：
文献类型：
--
作者：
Tashiro S;Handa T;Matsuda A;Ban T;Takigawa T;Miyasato K;Ishii K;Kugou K;Ohta K;Hiraoka Y;Masukata H;Kanoh J
染色体由结构和功能上不同的区域组成。然而，染色质结构和亚端粒（端粒邻近区域）功能形成的分子机制仍然不清楚。在这里，我们报道了保守的着丝粒蛋白Shugoshin 2 （Sgo2）在裂变酵母裂糖酵母（Schizosaccharomyces pombe）亚端粒染色质结构和功能中的作用。我们发现Sgo2在G2期优先定位于亚端粒，并且对于形成高度凝聚的亚端粒染色质体“旋钮”至关重要。此外，Sgo2的缺失导致亚端粒基因的抑制和亚端粒晚期起源的过早DNA复制。因此，由Sgo2组织的亚端粒特化染色质结构域抑制转录和复制，以确保适当的基因表达和复制时间。染色体由结构和功能上不同的区域组成。在这里，Tashiro等人报道了保守的着丝粒蛋白Sgo2在G2期优先定位于亚端粒，对于形成高度凝聚的亚端粒染色质体“knob”至关重要。
A chromosome is composed of structurally and functionally distinct domains. However, the molecular mechanisms underlying the formation of chromatin structure and the function of subtelomeres, the telomere-adjacent regions, remain obscure. Here we report the roles of the conserved centromeric protein Shugoshin 2 (Sgo2) in defining chromatin structure and functions of the subtelomeres in the fission yeast Schizosaccharomyces pombe. We show that Sgo2 localizes at the subtelomeres preferentially during G2 phase and is essential for the formation of a highly condensed subtelomeric chromatin body ‘knob'. Furthermore, the absence of Sgo2 leads to the derepression of the subtelomeric genes and premature DNA replication at the subtelomeric late origins. Thus, the subtelomeric specialized chromatin domain organized by Sgo2 represses both transcription and replication to ensure proper gene expression and replication timing. A chromosome is composed of structurally and functionally distinct domains. Here, Tashiro et al. report that the conserved centromeric protein Sgo2 localizes at the subtelomeres preferentially during G2 phase and is essential for the formation of a highly condensed subtelomeric chromatin body “knob”.