NOVEL FORMS OF NEURONAL MIGRATION IN THE RAT CEREBELLUM
NOVEL FORMS OF NEURONAL MIGRATION IN THE RAT CEREBELLUM
复制标题
DOI：
10.1002/jnr.490400209
复制
发表时间：
1995-02-01
影响因子：
4.2
通讯作者：
LIESI, P
中科院分区：
文献类型：
--
作者：
HAGER, G;DODT, HU;LIESI, P
用红外视频显微镜观察新生大鼠小脑（P0-P14）颗粒神经元迁移，观察时间长达24小时。最初（P0-P5），颗粒神经元沿着其他神经纤维的径向迁移路径移动。这些通路可能是由双极颗粒神经元建立的，双极颗粒神经元通过一个突起附着到外基底膜，并将另一个突起延伸到浦肯野细胞层。在P5-P8，大量颗粒神经元水平移动并延伸长的平行纤维。径向和水平迁移的颗粒神经元在其预先形成的过程中通过核移位移动，速度在6和120 μ m/hr之间变化。在P10-P12动物中，水平取向的颗粒神经元开始径向迁移。它们沿着其他神经纤维的放射状通路或与成熟的胶质突起接触进入内部颗粒细胞层。放射状神经元迁移途径消失，而胶质细胞的过程中保持并达到基板P14。这些结果描述了神经元迁移的新型径向和水平模式，其独立于物理胶质引导进行。(C)1995 Wiley-Liss，Inc.
Infrared video microscopy of neonatal rat cerebellum (P0-P14) was used to directly visualize migrating granule neurons in relation to other cerebellar cells in a brain slice for up to 24 hr. Initially (P0-P5), granule neurons move along radial migration pathways of other neuronal fibers. These pathways are probably established by the bipolar granule neurons that attach to the external basement membrane via one process and extend another process toward the Purkinje cell layer. At P5-P8, a substantial number of granule neurons move horizontally and extend long parallel fibers. Both radially and horizontally migrating granule neurons move by nuclear translocation inside their preformed processes with a speed that varies between 6 and 120 mu m/hr. In P10-P12 animals, the horizontally oriented granule neurons start to migrate radially. They move into the internal granule cell layer either along the radial pathways of other neuronal fibers or in contact with the matured glial processes. The radial neuronal migration pathways disappear by P14 whereas the glial cell processes are maintained and reach the basal lamina. These results describe novel radial and horizontal modes of neuronal migration that proceed independently of the physical glial guidance. (C) 1995 Wiley-Liss, Inc.