FREQUENCY OF CILIARY BEAT MEASURED WITH A PHOTO-SENSITIVE CELL
FREQUENCY OF CILIARY BEAT MEASURED WITH A PHOTO-SENSITIVE CELL
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DOI：
10.1038/196592a0
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发表时间：
1962-01-01
期刊：
影响因子：
64.8
通讯作者：
RYLANDER, R
中科院分区：
文献类型：
--
作者：
DALHAMN, T;RYLANDER, R
Rivera1对纤毛的现有知识进行了综述。不同的方法已经被用来估计频率和测试不同物质对纤毛搏动的影响。一种常见的方法是将煤和种子等颗粒滴在有纤毛的上皮细胞上，并计算颗粒行驶一定距离所需的时间。这种方法给出了纤毛速度的粗略值，但其他因素，如粘液层的粘度和厚度，严重干扰了结果。‘Flimmeruhr’3和‘Cilioscribe’4是早期作者描述的其他方法。借助于Leitz Ultra-Pak垂直光学显微镜，发展了更好的测量方法。电影记录和频闪检查结合了更高的准确性和测量时对纤毛的较小创伤影响。Dalhamn系统地研究了这两种方法，并描述了一种电影摄影设备，它可以确定活体兔子纤毛跳动频率的非常微小的变化。频闪观察法受到了相当严厉的批评，笔者并不推荐。在组织培养中对纤毛上皮小碎片的振动活性进行显微镜计数是另一种用于测试各种药剂的影响的方法。电影摄影法的一个缺点是不能立即获得结果，而且由于不可能进行连续测量，被测试物质的轻微影响可能被忽略。
A REVIEW of the present knowledge of cilia has been made by Rivera1. Various methods have been used for estimating the frequency and testing the effects of different substances on ciliary beat. A common method has been to drop particles such as coal and seed on ciliated epithelium and to count the time required for the particle to travel a certain distance2. This method gives a rough value for the speed of the cilia, but other factors such as viscosity and thickness of the mucous layer interfere severely with the results. The ‘Flimmeruhr’3and the ‘Cilioscribe’4were other methods described by earlier authors. With the aid of the Leitz Ultra-Pak vertical light microscope better measuring methods have been developed. Cinematographic recording and stroboscopy combine greater accuracy with less traumatic effect on the cilia when measuring. Dalhamn examines the two methods systematically5and describes a cinematographic equipment which makes it possible to determine quite small changes in frequency of ciliary beat in living rabbits6. The strobosoopic method is rather severely criticized and not recommended by this author. Microscopic counting of the vibratory activity of small ciliated epithelium fragments in tissue cultures is another method which has been used for testing the influence of various agents7. A disadvantage of the cinematographic method is that the results are not available immediately, and, as continuous measurement is not possible, minor effects of substances tested might be overlooked.