Real-Time Vital Mineralization Detection and Quantification during In Vitro Osteoblast Differentiation.
Real-Time Vital Mineralization Detection and Quantification during In Vitro Osteoblast Differentiation.
复制标题
实时重要矿化检测和体外成骨细胞分化过程中的定量。
DOI：
10.1186/s12575-018-0079-4
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发表时间：
2018
影响因子：
6.4
通讯作者：
Myklebost O
中科院分区：
文献类型：
--
作者：
Serguienko A;Wang MY;Myklebost O
在成骨细胞的体外研究中，传统上使用茜素红作为检测和定量矿化的金标准，矿化是成骨细胞分化的标志。然而，这种方法存在许多缺点，包括需要固定细胞，这阻碍了额外的测量。几年前，Calcein Green被提议作为茜素红的替代品，其优点是可以在活细胞中直接检测到。然而，该方案仍然很耗时，而且它从未成功取代茜素红。现在，有了更高效的成像系统，我们提出了一种使用Calcein Green的方案，它提供了显著的优势。在整个分化期的任何时间点，以最小的时间花费，有效地检测和准确地实时量化成骨细胞矿化。Calcein Green与IncuCyte ZOOM实时成像站的结合可以有效地取代茜素红方法，并且可以非常准确和节省时间地评估基质矿化的水平和动态。本文的在线版本（10.1186/s12575-018-0079-4）包含补充材料，授权用户可以使用。
In vitro studies of osteoblasts traditionally use Alizarin Red as a golden standard for the detection and quantification of mineralization, which is a marker of osteoblast differentiation. However, this method presents a number of drawbacks, including the need to fix cells, which prevents additional measurements. Years ago, Calcein Green was proposed as an alternative to Alizarin Red, with the advantage to be directly detectable in live cells. However, the protocol was still time-consuming, and it never managed to replace Alizarin Red. Now, with more efficient imaging systems, we present a protocol using Calcein Green which provides significant advantages. The osteoblast mineralization was efficiently detected and accurately quantified in real time at any desired time point across the entire differentiation period, with a minimum time expenditure. The combination of Calcein Green and the real-time imaging station IncuCyte ZOOM can efficiently replace the Alizarin Red method, and allows very accurate and time-saving assessment of the level and the dynamics of matrix mineralization. The online version of this article (10.1186/s12575-018-0079-4) contains supplementary material, which is available to authorized users.
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影响因子：
4
作者：
Serguienko, Anastassia;Hanes, Robert;Munthe, Else
通讯作者：
Munthe, Else
影响因子：
4.2
作者：
Uchimura, E;Machida, H;Ohgushi, H
通讯作者：
Ohgushi, H
影响因子：
2.7
作者：
Du, SJ;Frenkel, V;Zohar, Y
通讯作者：
Zohar, Y
影响因子：
5.2
作者：
Hakelien, Anne-Mari;Bryne, Jan Christian;Meza-Zepeda, Leonardo A.
通讯作者：
Meza-Zepeda, Leonardo A.