Müllerian inhibiting substance in sex-reversed dogs.
Müllerian inhibiting substance in sex-reversed dogs.
复制标题
性逆转狗中的苗勒氏管抑制物质。
DOI：
10.1095/biolreprod37.4.1015
复制
发表时间：
1987
影响因子：
3.6
通讯作者：
Patterson,DF
中科院分区：
文献类型：
--
作者：
Meyers-Wallen,VN;Donahoe,PK;Manganaro,T;Patterson,DF
在正常男性中，胚胎关键期睾丸产生的苗勒管抑制物质（MIS）被认为会诱导苗勒管系统（包括输卵管和子宫）的退化。在XX性反转的狗中，有一个明显的矛盾被报道：在睾丸或卵睾丸存在的情况下，子宫仍然存在。本研究的目的是确定XX雄性犬的睾丸和真雌雄同体犬的卵睾丸是否产生MIS，并检查受累犬苗勒管衍生物的解剖结构，以寻找退化的证据。在生物测定中检测性腺样品的MIS活性。XX男性的MIS活动评分与正常XY男性相似，但明显高于正常XX女性。XX真两性人的MIS活动评分介于正常XX女性和XY男性之间。在真两性组中，睾丸组织比例≥ 1/2的卵睾丸MIS评分高于睾丸组织比例< 1/2的卵睾丸。XX雄性动物的每个睾丸附近都有发育良好的附睾，但没有输卵管。在真两性体中，当相邻的卵睾丸≥ 1/2为睾丸时，输卵管退化，出现附睾，且该性腺中MIS活性高。少数具有中等水平MIS活性的卵睾丸同时具有输卵管和附睾。苗勒管系统的输卵管部分的回归与睾丸组织的数量和性腺的MIS活性呈正相关，正如Jost最初的假设所预测的那样。然而，在所有性反转犬中，无论睾丸组织的数量或MIS活性水平如何，子宫均完整存在且大小正常。这一发现表明苗勒管系统的子宫和输卵管部分对MIS的敏感性可能不同，或者子宫部分的消退并不严格依赖于MIS的浓度。
In normal males, Müllerian Inhibiting Substance (MIS), produced by testes during an embryonic critical period, is thought to induce regression of the Müllerian duct system, including the oviducts and uterus. In XX sex-reversed dogs, an apparent contradiction has been reported: The uterus persists in the presence of testes or ovotestes. The objective of this study is to determine whether testes of XX male and ovotestes of true hermaphrodite dogs produce MIS, and to examine the anatomy of Müllerian duct derivatives of affected dogs for evidence of regression. Gonadal samples were tested for MIS activity in a bioassay. The mean MIS activity score of XX males was similar to that of normal XY males and significantly > that of normal XX females. The mean MIS activity score of XX true hermaphrodites was intermediate between normal XX females and XY males. Within the true hermaphrodite group, ovotestes in which the proportion of testicular tissue was ≥ 1/2 had higher MIS scores than those in which the proportion of testicular tissue was < 1/2. XX males had a well-developed epididymis adjacent to each testis, but no oviducts. In true hermaphrodites, the oviduct regressed and an epididymis was present when ≥ 1/2 of the adjacent ovotestis was testicular, and MIS activity in that gonad was high. A few ovotestes with intermediate levels of MIS activity had both an oviduct and an epididymis. Regression of the oviductal portion of the Müllerian duct system was positively correlated to the amount of testicular tissue and the MIS activity of the gonad, as would be predicted by Jost’s original hypothesis. However, in all sex-reversed dogs, regardless of the amount of testicular tissue or level of MIS activity, the uterus was present in its entirety and was of normal size. This finding suggests that the uterine and oviductal portions of the Müllerian duct system may differ in their sensitivity to MIS or that regression of the uterine portion is not strictly dependent upon the concentration of MIS.