Abundance and diversity of ammonia-oxidizing archaea and bacteria in the rhizosphere soil of three plants in the Ebinur Lake wetland
Abundance and diversity of ammonia-oxidizing archaea and bacteria in the rhizosphere soil of three plants in the Ebinur Lake wetland
复制标题
艾比湖湿地三种植物根际土壤氨氧化古菌和细菌的丰度和多样性
DOI：
10.1139/cjm-2016-0492
复制
发表时间：
2017-07-01
影响因子：
2.8
通讯作者：
Gao, Yan
中科院分区：
文献类型：
--
作者：
He, Yuan;Hu, Wenge;Gao, Yan
氨氧化是由氨氧化细菌(AOB)和氨氧化古细菌(AOA)进行的。艾比湖湿地是中国温带干旱区湿地生态系统的最佳代表。从根际土壤和非根际土壤中采集土壤样品，包括盐碱草(H和H‘)、芦荟(R和R’)和卡瑞林(K和K‘)，以研究环境因子与AOB和AOA群落结构的关系。系统发育分析表明，AOA序列属于Nitrosopumilus和Nitrossphaera集群。AOB归入Nitrosspira sp.和亚硝酸单胞菌(NitroSomonas sp.)定量聚合酶链式反应结果表明，AOA丰度在2.09×10(4)~2.94×10(5)个基因拷贝/g土壤之间。AOB丰度变化范围为2.91×10(5)~1.05×10(6)个/g土壤，高于AOA。冗余度分析表明，电导率、pH和NH4+-N可能影响AOA和AOB的群落结构。由于新疆艾比湖湿地AOB具有较高的多样性和丰度，AOB在氨氧化中的作用可能比AOA更重要。
Ammonia oxidation is carried out by ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA). The Ebinur Lake wetland is the best example of a temperate arid zone wetland ecosystem in China. Soil samples were collected from rhizosphere and non-rhizosphere soil containing Halocnemum strobilaceum (samples H and H'), Phragmites australis (samples R and R'), and Karelinia caspia (samples K and K') to study the relationship between environmental factors and the community structure of AOB and AOA. Phylogenetic analysis showed that the AOA sequences belonged to the Nitrosopumilus and Nitrososphaera clusters. AOB were grouped into Nitrosospira sp. and Nitrosomonas sp. Quantitative polymerase chain reaction results showed that the AOA abundance ranged from 2.09 x 10(4) to 2.94 x 10(5) gene copies/g soil. The highest number of AOA was detected in sample K, followed by samples R and H. AOB abundance varied between 2.91 x 10(5) and 1.05 x 10(6) gene copies/g soil, which was higher than that of AOA. Redundancy analysis indicated that electrical conductivity, pH, and NH4+-N might influence the community structure of AOA and AOB. AOB might play a more crucial role than AOA in ammonia oxidation based on AOB's higher diversity and abundance in the Ebinur Lake wetland in Xinjiang.