Yeast as a model host to dissect functions of viral and host factors in tombusvirus replication
Yeast as a model host to dissect functions of viral and host factors in tombusvirus replication
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DOI：
10.1016/j.virol.2005.09.017
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发表时间：
2006-01-05
期刊：
影响因子：
3.7
通讯作者：
Pogany, J
中科院分区：
文献类型：
--
作者：
Nagy, PD;Pogany, J
RNA复制是正链RNA病毒感染周期的中心过程。酵母作为模式宿主的发展和强大的体外分析与纯化的复制酶复合体，加上反向遗传方法，使番茄病毒，小植物RNA病毒，研究病毒RNA复制的基本方面的优秀系统。因此，体外方法已经导致了对复制和组装功能性病毒复制酶复合体的模板选择至关重要的蛋白质RNA相互作用的鉴定。此外，全基因组方法和蛋白质组学分析已经确定了一组影响番茄病毒复制的新宿主蛋白。总体而言，番茄病毒复制的快速进展揭示了病毒-宿主相互作用的耐人寻味和复杂的性质，这使得番茄病毒的强大复制成为可能。所获得的知识可能会刺激新的抗病毒方法以及其他方法的发展，这些方法可以使番茄病毒在生物技术应用中成为有用的工具。(C)2005 Elsevier Inc.保留所有权利。
RNA replication is the central process during the infectious cycles of plus-stranded RNA viruses. Development of Yeast as a model host and powerful in vitro assays with purified replicase complexes, together with reverse genetic approaches make tombusviruses, small plant RNA viruses, excellent systems to study fundamental aspects of viral RNA replication. Accordingly, in vitro approaches have led to the identification of protein RNA interactions that are essential for template selection for replication and assembly of the functional viral replicase complexes. Moreover, genome-wide approaches and proteomics analyses have identified a new set of host proteins that affected tombusvirus replication. Overall, rapid progress in tombusvirus replication has revealed intriguing and complex nature of virus-host interactions, which make robust replication of tombusviruses possible. The knowledge obtained will likely stimulate development of new antiviral methods as well as other approaches that could make tombusviruses useful tools in biotechnological applications. (c) 2005 Elsevier Inc. All rights reserved.