Keratinocyte K+ channels mediate Ca2+-induced differentiation.
Keratinocyte K+ channels mediate Ca2+-induced differentiation.
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角质形成细胞 K 通道介导 Ca2 诱导的分化。
DOI：
10.1111/1523-1747.ep12292585
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Pappone,PA
中科院分区：
文献类型：
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作者：
Mauro,T;Dixon,DB;Komuves,L;Hanley,K;Pappone,PA
K+通道激活与许多细胞的生长或分化有关。我们以前已经确定了一个70 pS的K+通道，发现只在分化的外皮蛋白阳性细胞。在这项研究中，我们研究的作用K+通道在钙离子诱导的角质形成细胞分化。与我们以前的报告一致，我们发现，K+电导开发仅在细胞培养在高细胞外Ca 2+。此外，Charybdotoxin或维拉帕米阻断这些K+通道，抑制Ca2+诱导的分化，作为评估的corpus包膜形成或转氨酶活性。这些结果表明，K+通道激活是必要的钙诱导分化。最后，我们用125I标记的Charybdotoxin证明了在完整的人类和小鼠表皮、毛囊和外分泌腺中存在K+通道，表明这些通道在体外和体内的角质形成细胞中都存在。因此，K+通道可能会缓和更多分化的角质形成细胞中的Ca2+内流，并可能在角质形成细胞分化中发挥核心作用。
K+channel activation has been associated with growth or differentiation in many cells. We have previously identified a 70-pS K+channel that was found only in differentiated involucrin-positive cells. In this study we examined the role of K+channels in Ca2+-induced keratinocyte differentiation. Consistent with our previous report, we found that a K+conductance developed only in cells cultured in high extracellular Ca2+. Addition of charybdotoxin or verapamil blocked these K+channels and inhibited Ca2+-induced differentiation, as assessed by cornified envelope formation or transglutaminase activity. These results suggest that K+channel activation is necessary for Ca2+-induced differentiation. Finally, we used125I-labeled charybdotoxin to demonstrate the presence of K+channels in intact human and mouse epidermis, hair follicles, and eccrine glands, indicating that these channels are found in keratinocytes bothin vitroandin vivo. Thus K+channels may moderate Ca2+influx in more differentiated keratinocytes and may play a central role in keratinocyte differentiation.