Localization of acid phosphatase in Saccharomyces cerevisiae: a clue to cell wall formation
Localization of acid phosphatase in Saccharomyces cerevisiae: a clue to cell wall formation
复制标题
酿酒酵母中酸性磷酸酶的定位：细胞壁形成的线索
作者：
W. Linnemans;P. Boer;P. Elbers
酸性磷酸酶存在于酿酒酵母的两层细胞包膜中。它们被另一层分离，该层不含酸性磷酸酶。我们有证据表明，细胞壁是由两个独立的阶段组成的。在第一阶段，细胞壁在芽的形成过程中形成。葡聚糖酶囊泡参与了这一过程。在第二阶段，一个厚层被沉积在新的细胞壁的内侧。这导致成熟酵母细胞的壁厚而坚硬。这后一层可能是组装在质膜的外表面上。
Acid phosphatase is present in two layers of the cell envelope of Saccharomyces cerevisiae. These are separated by another layer, which is free of acid phosphatase. We have evidence that the cell wall is built up in two stages, which are independent. In the first stage, the cell wall is built up during the formation of the bud. Glucanase vesicles are involved in this process. In the second stage, a thick layer is deposited at the inside against the new cell wall. This results in the thick, rigid wall of the mature yeast cell. This latter layer is probably assembled on the outer surface of the plasmalemma.