Gene Rpi-bt1 from Solanum bulbocastanum Confers Resistance to Late Blight in Transgenic Potatoes
Gene Rpi-bt1 from Solanum bulbocastanum Confers Resistance to Late Blight in Transgenic Potatoes
复制标题
DOI：
10.1007/s12230-009-9100-4
复制
发表时间：
2009-11-01
影响因子：
1.5
通讯作者：
Belknap, W. R.
中科院分区：
文献类型：
--
作者：
Oosumi, T.;Rockhold, D. R.;Belknap, W. R.
致病疫霉是马铃薯晚疫病的病原菌，是世界范围内栽培马铃薯最具破坏性的病原菌。利用图谱克隆;从野生马铃薯种Solanumbulbocastanum中分离含有一簇六个核苷酸结合位点-富含亮氨酸的重复抗性基因类似物的基因组区域。四个基因是假基因，编码序列被移码突变或过早终止密码子中断。然而，当引入易感马铃薯时，这两个不间断的基因都不赋予对致病疫霉的抗性。用假基因特异性引物从致病疫霉感染的沙门氏菌中扩增出不间断的cDNA。球栗叶从抗晚疫病的球栗-马铃薯渐渗系（Rpi-bt 1）中扩增出相应的gDNA。在全植物温室测定中，发现在组成型马铃薯Ubi 3启动子的转录控制下的Rpi-bt 1基因在几个转基因马铃薯品系中赋予对致病疫霉的抗性。
Phytophthora infestans, the causal agent of late blight is the most devastating pathogen of cultivated potato worldwide. Utilizing map based cloning; a genomic region containing a cluster of six nucleotide binding site-leucine-rich repeat resistance gene analogs was isolated from the wild potato species Solanum bulbocastanum. Four genes were pseudogenes, with coding sequences interrupted by either frame shift mutations or premature stop codons. However, neither of the two uninterrupted genes conferred resistance to P. infestans when introduced into susceptible potatoes. Specific primers for one of the pseudogenes were used to amplify an uninterrupted cDNA from P. infestans-infected S. bulbocastanum leaves. A corresponding gDNA was amplified from a late blight-resistant bulbocastanum-tuberosum introgression line (Rpi-bt1). The Rpi-bt1 gene under transcriptional control of the constitutive potato Ubi3 promoter was found to confer resistance to P. infestans in several transgenic potato lines in a whole plant greenhouse assay.