Coxsackievirus B3-Associated Aseptic Meningitis: An Emerging Infection in Hong Kong
Coxsackievirus B3-Associated Aseptic Meningitis: An Emerging Infection in Hong Kong
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DOI：
10.1002/jmv.21998
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发表时间：
2011-03-01
影响因子：
12.7
通讯作者：
Lim, Wilina W. L.
中科院分区：
文献类型：
--
作者：
Wong, Ann H.;Lau, C. S.;Lim, Wilina W. L.
肠道病毒感染是儿童常见病，常合并无菌性脑膜炎。在2008年夏季，实验室监测发现香港的柯萨奇病毒B3相关无菌性脑膜炎的人数有所增加，占受感染人数的11.6%。本研究以EV在香港的流行情况为参考，分析过去10年CVB 3在香港的流行病学、流行模式及临床表现。使用人横纹肌肉瘤、人喉癌（HEp 2-C）、人肺成纤维细胞（MRC-5）和非洲绿色猴肾（Vero）细胞系，从呼吸道、脑脊液（CSF）、粪便和水泡样本中分离肠道病毒（EV）。通过使用单克隆抗体（mAB）的间接免疫荧光（IF）、中和试验以及部分VP 1测序对病毒分离株进行鉴定和表征。与往年不同的是，IF检测结果显示，2008年的大多数分离株不能用mAB分型，提示抗原性发生了变化。序列分析表明，这些菌株与中国阜阳的新分离物聚在一起。回顾1999年至2008年的数据显示，CVB 3在2005年和2008年的活性增加，并且这2年的分离株显示VP 1基因的密码子277处的氨基酸由苏氨酸变为丙氨酸，这可能与中枢神经系统（CNS）疾病相关。医学病毒学杂志83：483- 489，2011。(C)2011 Wiley-Liss，Inc.
Enterovirus (EV) infection is a common disease of childhood and associated not uncommonly with aseptic meningitis. In the summer of 2008, laboratory surveillance has detected increased number of coxsackievirus B3 (CVB3) associated aseptic meningitis in Hong Kong, constituting 11.6% of those infected. This study analyzed the epidemiology, circulating pattern, and clinical presentations of CVB3 in Hong Kong over the last 10 years with reference to the circulation of EV in the locality. Enteroviruses (EV) were isolated from respiratory, cerebrospinal fluid (CSF), stool, and vesicular samples using human rhabdomyosarcoma, human laryngeal carcinoma (HEp2-C), human lung fibroblast (MRC-5), and African green monkey kidney (Vero) cell lines. Virus isolates were identified and characterized by indirect immunofluorescence (IF) using monoclonal antibodies (mAB), neutralization test as well as partial VP1 sequencing. Different from previous years, IF test result showed that majority of the isolates from 2008 were untypeable by the mAB suggesting antigenic change. Sequence analysis revealed that these isolates were clustered with recent isolate from Fuyang, China. Review of data from 1999 to 2008 showed increased activity of CVB3 in the years 2005 and 2008, and isolates in these 2 years displayed an amino acid change from threonine to alanine at codon 277 of the VP1 gene, which may be associated with central nervous system (CNS) disease. J. Med. Virol. 83:483-489,2011. (C) 2011 Wiley-Liss, Inc.