Depolarization‐dependent survival of cultured mouse cerebellar granule neurons is strain‐restrained
Depolarization‐dependent survival of cultured mouse cerebellar granule neurons is strain‐restrained
复制标题
培养的小鼠小脑颗粒神经元的去极化依赖性存活受到应变抑制
DOI：
--
复制
发表时间：
2000
影响因子：
3.4
通讯作者：
A. Ogura
中科院分区：
文献类型：
--
作者：
N. Fujikawa;K. Tominaga;M. Okabe;A. Ogura
从大鼠分离的小脑颗粒神经元（CGNs）经常被用作分析神经元活性依赖性存活的模型系统。如果不向培养基中添加去极化剂（氯化钾或谷氨酸盐），这些细胞不能在培养中存活。然而，据报道，小鼠CGN的行为有所不同。在这里，我们发现小鼠CGN存活所需的去极化是品系依赖的，这可能对未来使用转基因动物进行分析很重要。Balb/C小鼠的CGN在不添加KCl的情况下可以存活，而C57Bl/6小鼠的CGN则不能存活（类似于大鼠的CGN）。Balb/C小鼠cgn的促生存活性可通过共培养转移。然而，Balb/C小鼠CGN培养的培养基无效，说明预期释放的神经营养物质是不稳定的。虽然我们还没有确定具体的物质，但它可能独立于神经营养素。在生产转基因动物时，有必要选择小鼠品系来分析活性依赖性神经元的存活。
Cerebellar granule neurons (CGNs) isolated from the rat are often used as a model system for the analysis of activity‐dependent survival of neurons. These cells do not survive in culture without addition of a depolarizing agent (KCl or glutamate) to the medium. However, it has been reported that mouse CGN behave differently. Here we found that the requirement for depolarization for the survival of the mouse CGN was strain‐dependent, which may be important for future analyses using transgenic animals. CGNs from the Balb/C mouse could survive without KCl addition, whereas CGNs from the C57Bl/6 mouse could not (similar to the rat CGN). The survival‐promoting activity of the Balb/C mouse CGNs was transferable by coculturing. However, the medium of the Balb/C mouse CGN culture was ineffective, suggesting that the neurotrophic substance that is supposed to be released is labile. Although we are yet to specify the substance, it might be independent of neurotrophins. It is necessary to select the strain of mouse in the production of transgenic animals for the analyses of activity‐dependent neuronal survival.