Microscale topographic surfaces modulate three-dimensional migration of human spermatozoa.
Microscale topographic surfaces modulate three-dimensional migration of human spermatozoa.
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DOI：
10.1016/j.colsurfb.2020.111096
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发表时间：
2020-05
期刊：
影响因子：
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通讯作者：
Gui Huang;Sun Li;Xueqing Jin;Meng Qi;Xiangjun Gong;Guangzhao Zhang
中科院分区：
文献类型：
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作者：
Gui Huang;Sun Li;Xueqing Jin;Meng Qi;Xiangjun Gong;Guangzhao Zhang
精子在女性生殖道中的迁移对生殖是至关重要的。在这一过程中，表面形貌预计是一个至关重要的决定因素。利用数字全息显微镜（DHM），我们研究了人类精子在平面玻璃表面和由单层紧密堆积的二氧化硅胶体颗粒制成的具有可调粗糙度的微尺度地形表面附近的三维运动动力学。一般来说，粗糙的表面通过表面地形调节的水动力相互作用对精子的迁移产生负面影响，表现为游动方向分布更宽的振荡轨迹，3D速度降低，螺旋/超激活/超螺旋运动减少。然而，精子在平面玻璃表面和具有类似精子尾部特征维数的表面附近的运动略有不同。我们的研究为理解和操纵精子运动提供了新的见解。
Sperm migration in the female reproductive tract is vital for reproduction. Surface topography is expected to be a vital determinant on this process. Using digital holographic microscopy (DHM), we investigated three-dimensional (3D) motion dynamics of human spermatozoa near a flat glass surface and microscale topographic surfaces with tunable roughness fabricated by a monolayer of closely packed silica colloidal particles. Generally, the rougher surfaces show negative impacts on the sperm migration through the hydrodynamic interactions modulated by surface topography, reflected as oscillating trajectories with wider swimming orientation distribution, reduced 3D velocity and less helical/hyperactivated/hyerhelical motions. Nevertheless, slight difference is observed for the sperm motion near the flat glass surface and the surface with a feature dimension similar to the sperm tail. Our study provides new insights in understanding and manipulating sperm motions.