Differential gene expression in the distal tip endoderm of the embryonic mouse lung.
Differential gene expression in the distal tip endoderm of the embryonic mouse lung.
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DOI：
10.1016/s1567-133x(02)00057-1
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发表时间：
2002-12-01
影响因子：
1.2
通讯作者：
Hogan, Brigid L. M.
中科院分区：
文献类型：
--
作者：
Liu, Yuru;Hogan, Brigid L. M.
在小鼠肺发育的早期，一些编码信号分子的基因在远端芽的上皮和间充质中差异表达。有证据表明，它们在调控芽生长和分枝以及两个细胞层的近端-远端模式的刻板过程中发挥了作用。为了更好地了解分支形态发生的机制，采用消减杂交和差异筛选的方法，寻找在胚胎11.5天肺芽顶端的上皮细胞中优先表达的基因，而不是更近端的区域。鉴定了20个基因，根据序列分析将其归类为不同的类别，并通过整体安装原位杂交证实了它们的远端表达。
During the early development of the mouse lung a number of genes encoding signaling molecules are differentially expressed in the epithelium and mesenchyme of the distal buds. Evidence suggests they play a role in regulating the stereotypic processes of bud outgrowth and branching as well as proximal-distal patterning of both cell layers. To better understand the mechanisms underlying branching morphogenesis, a subtractive hybridization and differential screen was carried out for genes preferentially expressed in the epithelium at the tips of embryonic day 11.5 lung buds, versus more proximal regions. Twenty genes were identified, assigned to different categories based on sequence analysis, and their distal expression confirmed by whole-mount in situ hybridization.