Induction of gastric GIST in rat and establishment of GIST cell line
Induction of gastric GIST in rat and establishment of GIST cell line
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DOI：
10.1016/j.canlet.2005.02.046
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发表时间：
2006-01-18
期刊：
影响因子：
9.7
通讯作者：
Hattori, T
中科院分区：
文献类型：
--
作者：
Mukaisho, K;Miwa, K;Hattori, T
据报道，连续给予直接反应致癌物N-甲基-N '-硝基-N-亚硝基胍不仅在大鼠中产生腺癌，而且还产生间叶肿瘤。大量被诊断为胃肠道平滑肌肿瘤的肿瘤实际上代表了人类病例中的胃肠道间质瘤（GIST）。我们在大鼠中通过十二指肠反流诱导间叶肿瘤。为了阐明这些间叶性肿瘤的分化，进行了免疫组化研究。此外，本研究首次建立了由十二指肠返流诱导的GIST衍生的GIST-DR培养模型。GIST-DR细胞，在体外和体内，是强烈的免疫阳性的KIT和CD 34，和ST 1571（伊马替尼甲磺酸盐）阻断该细胞系的增殖。提示十二指肠返流在胃肠道间质瘤的组织发生中起一定作用。(c)2005爱思唯尔爱尔兰有限公司保留所有权利。
Continuous administration of the direct-reacting carcinogen N-methyl-N'-nitro-N-nitrosoguanidine reportedly produces not only adenocarcinoma in rats, but also mesenchymal tumors. A large number of tumors diagnosed as gastrointestinal smooth muscle tumors actually represent gastrointestinal stromal tumors (GISTs) in human cases. We have induced mesenchymal tumors by duodenal reflux in rats. To clarify the differentiation of these mesenchymal tumors, immunohistochemical investigations were undertaken. In addition, the first Culture model of GIST-DR derived from GIST induced by duodenal reflux was established. GIST-DR cells, both in vitro and in vivo, were strongly immunopositive for both KIT and CD34, and ST1571 (Imatinib mesylate) blocked the proliferation of this cell line. The present results Suggest that duodenal reflux plays a role in the histogenesis of GIST. (c) 2005 Elsevier Ireland Ltd. All rights reserved.