Separation and determination of the effective components in the alabastrum of Edgeworthia chrysantha Lindl. by micellar electrokinetic capillary chromatography.
Separation and determination of the effective components in the alabastrum of Edgeworthia chrysantha Lindl. by micellar electrokinetic capillary chromatography.
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DOI：
10.1016/j.jpba.2005.09.001
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发表时间：
2006-03
影响因子：
3.4
通讯作者：
Shufang Wang;Yiyu Cheng
中科院分区：
文献类型：
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作者：
Shufang Wang;Yiyu Cheng
建立了一种快速、高效的胶束电动毛细管色谱法（MEKC），用于测定鸡冠花花蕾中的鸡冠花苷C（1）、山奈酚-3-O-β-d-葡萄糖苷（2）和芦丁（3）。第一次研究了缓冲液、SDS、有机改性剂的浓度、表观pH值、外加电压和温度等因素对分离的影响。在25 kV和15°C下，使用含有25 mM Na 2B 4 O 7、30 mM NaH 2 PO 4、60 mM SDS和15%乙腈（pH*9.1）的电解质，分析物在15分钟内得到良好分离。在最佳条件下，待测物峰面积与相应浓度的相关系数为0.9976-0.9981。相对标准偏差（R.S.D.）的迁移时间和峰面积的范围分别为0.6-1.7和1.9- 5.3%。E.金花茶对不同地点采集的样品进行了测定，回收率为95.9%~ 104.3%。结果表明，两种真实的样品之间存在显著差异。
A rapid and efficient micellar electrokinetic capillary chromatography (MEKC) method was developed to analyze edgeworoside C (1), kaempferol-3-O-β-d-glucoside (2) and rutin (3) in the alabastrum of Edgeworthia chrysantha Lindl. for the first time. The factors that affect the separation were studied, such as the concentrations of the buffer, SDS, and organic modifier, the apparent pH, the applied voltage and temperature. The analytes were well separated within 15min with an electrolyte containing 25mM Na2B4O7, 30mM NaH2PO4, 60mM SDS and 15% acetonitrile (pH*9.1) at 25kV and 15°C. The correlation coefficients between the peak areas of analytes and the corresponding concentrations were 0.9976–0.9981 under the optimum conditions. The relative standard deviations (R.S.D.) of the migration time and peak area were in the range 0.6–1.7 and 1.9–5.3%, respectively. The contents of analytes in E. chrysantha Lindl. collected from the different places were successfully determined with the recoveries ranging from 95.9 to 104.3%. And, the results demonstrated that there was significant difference between the two real samples.