Preparation of spatially ordered multilayer thin films of antibody and their binding properties.
Preparation of spatially ordered multilayer thin films of antibody and their binding properties.
复制标题
空间有序多层抗体薄膜的制备及其结合特性。
DOI：
10.1016/s0956-5663(00)00113-5
复制
发表时间：
2000
影响因子：
12.6
通讯作者：
J. Anzai
中科院分区：
文献类型：
--
作者：
T. Hoshi;H. Saiki;J. Anzai
Spatially ordered multilayer thin films containing anti-fluoresceinisothiocyanate (anti-FITC) were prepared on the surface of a quartz slide to study the binding properties of the multilayer films. A quartz slide was treated in solutions of avidin and biotin-labeled anti-FITC alternately and repeatedly to form multilayer thin films through a strong affinity between avidin and biotin. A spectrophotometric study revealed explicitly that the thin films thus prepared consisted of alternate monomolecular layers of avidin and biotin-labeled anti-FITC. The antibody retained its binding activity to antigen in the multilayer thin film, though the antigen could not access the antibody embedded deep in the multilayer film. Only the outermost four or five layers of antibody were involved in the binding of antigen.
