Stress resistance of biofilm and planktonic Lactobacillus plantarum subsp plantarum JCM 1149
Stress resistance of biofilm and planktonic Lactobacillus plantarum subsp plantarum JCM 1149
复制标题
DOI：
10.1016/j.fm.2009.04.001
复制
发表时间：
2009-09-01
影响因子：
5.3
通讯作者：
Nomura, Nobuhiko
中科院分区：
文献类型：
--
作者：
Kubota, Hiromi;Senda, Shouko;Nomura, Nobuhiko
本研究的目的是研究生物膜和浮游食品腐败乳酸菌(LAB)对环境胁迫的抵抗力的变化。植物乳杆菌亚种生物膜和浮游细胞的应激反应以一株典型腐败菌Plantarum JCM 1149为研究对象，对其在不同有机酸(即冰醋酸、柠檬酸、乳酸和苹果酸)、乙醇和次氯酸钠中的存活情况进行了研究。无论是在稳定期还是在对数阶段，生物膜中的细菌细胞都比浮游细菌细胞对所有处理表现出更大的抵抗力。在细胞悬浮液稀释后，浮游细菌细胞对醋酸的抵抗力降低：然而，有趣的是，生物膜中的细菌细胞即使在悬浮液或细胞悬浮液被稀释后仍保持对醋酸的抵抗力。这些发现提示了来自生物膜的乳酸菌在食品中悬浮或稀释导致食品变质的风险，并证明了控制乳酸菌生物膜在食品工业中的重要性。(C)2009爱思唯尔有限公司。保留所有权利。
The purpose of this study was to investigate the change in resistance of biofilm and planktonic food spoilage lactic acid bacteria (LAB) to environmental stresses, which strongly inhibit bacterial growth and are important in food preservation or in disinfection. The stress responses of biofilm and planktonic cells of Lactobacillus plantarum subsp. plantarum JCM 1149, which was used as a model spoilage bacterium, in various organic acids (namely, acetic acid, citric acid, lactic acid, and malic acid), ethanol, and sodium hypochlorite, were investigated using survival tests. The bacterial cells in biofilms showed greater resistance to all treatments than the planktonic bacterial cells in either the stationary or logarithmic phase. The planktonic bacterial cells showed reduced resistance to acetic acid after the cell suspension was diluted: however, intriguingly, the bacterial cells in biofilms maintained their resistance to acetic acid even after they were suspended or the cell suspension was diluted. These findings suggested the risk for food spoilage due to LAB derived from biofilms and suspended or diluted in foods, and demonstrated the importance of controlling biofilms of LAB in the food industry. (C) 2009 Elsevier Ltd. All rights reserved.