A simplified biomolecule attachment strategy for biosensing using a porous Si oxide interferometer.
A simplified biomolecule attachment strategy for biosensing using a porous Si oxide interferometer.
复制标题
使用多孔氧化硅干涉仪进行生物传感的简化生物分子附着策略。
DOI：
10.1002/pssa.200674360
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Sailor,MichaelJ
中科院分区：
文献类型：
--
作者：
Perelman,LorenA;Schwartz,MichaelP;Wohlrab,AaronM;Vannieuwenhze,MichaelS;Sailor,MichaelJ
A simple strategy for linking biomolecules to porous Si surfaces and detecting peptide/drug binding is described. Porous Si is prepared using an electrochemical etch and then thermally oxidized by heating in ambient atmosphere. Bovine serum albumin (BSA) is then non‐covalently adsorbed to the inner pore walls of the porous Si oxide (PSiO2) matrix. The BSA layer is used as a linker for covalent attachment of the peptide Ac‐L‐Lysine‐D‐Alanine‐D‐Alanine (KAA) using published bioconjugation chemistry. BSA‐coated surfaces functionalized with KAA display specificity for the glycopeptide vancomycin while resisting adsorption of non‐specific reagents. While the biomolecule attachment strategy reported here is used to bind peptides, the scheme can be generalized to the linking of any primary amine‐containing molecule to PSiO2surfaces. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
