Effects of exogenous hexoses on bovine in vitro fertilized and cloned embryo development: Improved blastocyst formation after glucose replacement with fructose in a serum-free culture medium
Effects of exogenous hexoses on bovine in vitro fertilized and cloned embryo development: Improved blastocyst formation after glucose replacement with fructose in a serum-free culture medium
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DOI：
10.1002/mrd.10309
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发表时间：
2003-06-01
影响因子：
2.5
通讯作者：
Hwang, W
中科院分区：
文献类型：
--
作者：
Kwun, J;Chang, K;Hwang, W
为了评价三种己糖（葡萄糖、果糖和半乳糖）的胚胎营养作用，将来自体细胞核移植（SCNT）或体外受精（IVF）的牛胚胎培养在改良的合成输卵管液（mSOF）中，该液含有葡萄糖（1.5或5.6 mM）、果糖（1.5或5.6 mM）或半乳糖（1.5或5.6 mM）。与1.5 mM葡萄糖相比，使用1.5 mM果糖显著增强了SCNT（23 vs. 33%）和IVF胚胎（26 vs. 34%）的囊胚形成，而5.6 mM果糖没有改善囊胚形成。使用1.5 mM半乳糖没有改善SCNT胚胎的囊胚形成（22 vs. 23%），而它显著抑制IVF胚胎的囊胚形成（26 vs. 0%）。在SCNT和IVF胚胎中，5.6 mM葡萄糖或半乳糖显着抑制胚胎发育。在第二个实验中，在无葡萄糖的mSOF中，与0 mM果糖相比，浓度为0.75、1.5、3.0或5.6 mM的果糖能够支持桑椹胚（32-42 vs. 12%）和囊胚形成（30-38 vs. 12%）。在实验3中，添加果糖（1.5，3.0，或5.6 mM）mSOF含有1.5 mM葡萄糖没有进一步促进囊胚形成的SCNT胚胎相比，更换1.5 mM果糖。与1.5 mM葡萄糖相比，用1.5 mM果糖替代葡萄糖显著增加了囊胚中的总卵裂球（143 vs. 123个细胞）和滋养外胚层（TE）细胞（116 vs. 94个细胞），并降低了内细胞团（ICM）与TE细胞的比率（0.24 vs. 0.31）。1.5 mM果糖和葡萄糖的联合添加显著增加了ICM细胞数量（36.7个细胞）和ICM/TE比率（0.46）。总之，果糖可能是一种比葡萄糖更有效的能量底物，用于产生大量的SCNT衍生的可转移囊胚。摩尔Reprod. Dev. (C)2003 Wiley-Liss，Inc.
To evaluate the embryotrophic role of three hexoses (glucose, fructose, and galactose), bovine embryos derived from somatic cell nuclear transfer (SCNT) or in vitro-fertilization (IVF) were cultured in a modified synthetic oviductal fluid (mSOF), which contained either glucose (1.5 or 5.6 mM), fructose (1.5 or 5.6 mM), or galactose (1.5 or 5.6 mM). Compared to 1.5 mM glucose, use of 1.5 mM fructose significantly enhanced blastocyst formation in both SCNT (23 vs. 33%) and IVF embryos (26 vs. 34%), while 5.6 mM fructose did not improve blastocyst formation. Using 1.5 mM galactose did not improve blastocyst formation in SCNT embryos (22 vs. 23%), whereas it significantly inhibited blastocyst formation in IVF embryos (26 vs. 0%). In both SCNT and IVF embryos, 5.6 mM glucose or galactose significantly inhibited embryo development. In a second experiment, in glucose-free mSOF, fructose at concentrations of 0.75, 1.5, 3.0, or 5.6 mM was able to support to morula (32-42 vs. 12%) and blastocyst formation (30-38 vs. 12%) compared to 0 mM fructose. In Experiment 3, addition of fructose (1.5, 3.0, or 5.6 mM) to mSOF containing 1.5 mM glucose did not further promote blastocyst formation in SCNT embryos compared with replacement with 1.5 mM fructose only. Replacement of glucose with 1.5 mM fructose significantly increased total blastomeres (143 vs. 123 cells) and trophectodermal (TE) cells (116 vs. 94 cells) and decreased inner cell mass (ICM) to TE cell ratio (0.24 vs. 0.31) in blastocysts, compared to 1.5 mM glucose. The combined addition of 1.5 mM fructose and glucose significantly increased ICM cell number (36.7 cells) and ICM/TE ratio (0.46). In conclusion, fructose might be a more efficient energy substrate than glucose for producing large number of transferable blastocysts derived from SCNT. Mol. Reprod. Dev. (C) 2003 Wiley-Liss, Inc.