Neural and behavioral control in Caenorhabditis elegans by a yellow-light?activatable caged compound
Neural and behavioral control in Caenorhabditis elegans by a yellow-light?activatable caged compound
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黄光激活笼状化合物对秀丽隐杆线虫的神经和行为控制
DOI：
10.1073/pnas.2009634118
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Urano Yasuteru
中科院分区：
文献类型：
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作者：
Takahashi Hironori;Kamiya Mako;Kawatani Minoru;Umezawa Keitaro;Ukita Yoshiaki;Niwa Shinsuke;Oda Toshiyuki;Urano Yasuteru
秀丽隐杆线虫作为模型系统，以了解行为的神经基础，但应用笼化合物来操纵和监测的神经活动受到阻碍的先天natural hobic响应的短波长光或低的时间分辨率的光控线虫。在这里，我们开发了硼二吡咯亚甲基（BODIPY）衍生的笼状化合物，在黄色波长范围内的照明时释放生物活性的苯酚衍生物。我们发现，激活的瞬时受体电位香草素1（TRPV 1）阳离子通道的TRPV 1激动剂N-vanillylnonanamide在580 nm的空间有针对性的光学uncaging调制神经活动。此外，神经元激活照明诱导uncaging使光学控制的行为自由movingC。elegans而不诱导嗜热反应，并且在神经活动的解开和同时荧光监测之间没有串扰。
Caenorhabditis elegansis used as a model system to understand the neural basis of behavior, but application of caged compounds to manipulate and monitor the neural activity is hampered by the innate photophobic response of the nematode to short-wavelength light or by the low temporal resolution of photocontrol. Here, we develop boron dipyrromethene (BODIPY)-derived caged compounds that release bioactive phenol derivatives upon illumination in the yellow wavelength range. We show that activation of the transient receptor potential vanilloid 1 (TRPV1) cation channel by spatially targeted optical uncaging of the TRPV1 agonistN-vanillylnonanamide at 580 nm modulates neural activity. Further, neuronal activation by illumination-induced uncaging enables optical control of the behavior of freely movingC. eleganswithout inducing a photophobic response and without crosstalk between uncaging and simultaneous fluorescence monitoring of neural activity.