MiR-133b is frequently decreased in gastric cancer and its overexpression reduces the metastatic potential of gastric cancer cells.
MiR-133b is frequently decreased in gastric cancer and its overexpression reduces the metastatic potential of gastric cancer cells.
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MiR-133b在胃癌中经常减少，其过度表达降低了胃癌细胞的转移潜力
DOI：
10.1186/1471-2407-14-34
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发表时间：
2014-01-21
期刊：
影响因子：
3.8
通讯作者：
Zhu Z
中科院分区：
文献类型：
--
作者：
Zhao Y;Huang J;Zhang L;Qu Y;Li J;Yu B;Yan M;Yu Y;Liu B;Zhu Z
越来越多的证据表明，microRNAs参与了胃癌的发生和发展。在这里，我们研究miR-133b在胃癌中的作用。对140例胃癌组织和8株胃癌细胞株进行了实时荧光定量聚合酶链式反应(RT-PCR)分析。通过划痕实验、跨膜迁移和基质侵袭实验检测miR-133b对胃癌细胞转移的影响。在小鼠腹膜肿瘤模型上观察了miR-133b的体内作用。用生物信息学工具预测miR-133b的靶点，并通过荧光素酶报告分析、Western印迹和实时定量PCR进行验证。在70%(98/140)的胃癌患者中，miR-133b表达显著下调。MiR-133b的表达与胃癌的淋巴转移呈负相关。同样，miR-133b在7个受试胃癌细胞系中的表达显著低于永生化的非癌GES-1胃上皮细胞。MiR-133b过表达可显著抑制胃癌细胞的体内外转移。此外，转录因子Gli1被确定为miR-133b的直接靶标。MiR-133b可降低Gli1蛋白表达水平，但不影响Gli1蛋白表达水平。MiR-133b可显著降低胃癌细胞中含有Gli13‘非翻译区的荧光素酶活性。Gli1靶基因OPN和ZEB2也被miR133b抑制。MIR-133b在胃癌中经常降低。MiR-133b过表达可直接抑制Gli1蛋白的表达，从而抑制细胞的体外和体内转移。提示miR-133b在胃癌转移中起重要作用。
Emerging evidence has shown that microRNAs are involved in gastric cancer development and progression. Here we examine the role of miR-133b in gastric cancer. Quantitative real-time PCR analysis was performed in 140 patient gastric cancer tissues and 8 gastric cancer cell lines. The effects of miR-133b in gastric cancer cells metastasis were examined by scratch assay, transwell migration and matrigel invasion. In vivo effects of miR-133b were examined in an intraperitoneal mouse tumor model. Targets of miR-133b were predicted by bioinformatics tools and validated by luciferase reporter analyses, western blot, and quantitative real-time PCR. MiR-133b was significantly downregulated in 70% (98/140) of gastric cancer patients. Expression of miR-133b was negatively correlated with lymph node metastasis of gastric cancer in patients. Similarly, the expression of miR-133b was significantly lower in seven tested gastric cancer cell lines than in the immortalized non-cancerous GES-1 gastric epithelial cells. Overexpression of miR-133b markedly inhibited metastasis of gastric cancer cells in vitro and in vivo. Moreover, the transcriptional factor Gli1 was identified as a direct target for miR-133b. Level of Gli1 protein but not mRNA was decreased by miR-133b. Activity of luciferase with Gli1 3′-untranslated region was markedly decreased by miR-133b in gastric cancer cells. Gli1 target genes, OPN and Zeb2, were also inhibited by miR133b. MiR-133b is frequently decreased in gastric cancer. Overexpression of miR-133b inhibits cell metastasis in vitro and in vivo partly by directly suppressing expression of Gli1 protein. These results suggested that miR-133b plays an important role in gastric cancer metastasis.
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影响因子：
4.8
作者：
Das, Shamik;Harris, Lillianne G.;Shevde, Lalita A.
通讯作者：
Shevde, Lalita A.
DOI：
10.1111/j.1440-1746.2008.05666.x
发表时间：
2009-04-01
影响因子：
4.1
作者：
Guo, Junming;Miao, Ying;Wang, Yanjun
通讯作者：
Wang, Yanjun
影响因子：
4.8
作者：
Ji, Zhenyu;Mei, Fang C.;Cheng, Xiaodong
通讯作者：
Cheng, Xiaodong
影响因子：
64.5
作者：
Lewis, BP;Shih, IH;Burge, CB
通讯作者：
Burge, CB
影响因子：
56.9
作者：
Lim, LP;Glasner, ME;Bartel, DP
通讯作者：
Bartel, DP