Antisense oligonucleotide-mediated inhibition of metallothionein protein synthesis in neuroblastoma IMR 32 and Chang liver cells in culture.
Antisense oligonucleotide-mediated inhibition of metallothionein protein synthesis in neuroblastoma IMR 32 and Chang liver cells in culture.
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DOI：
10.1159/000109311
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发表时间：
1992-03
期刊：
影响因子：
--
通讯作者：
P. Iversen;M. Ebadi
中科院分区：
文献类型：
--
作者：
P. Iversen;M. Ebadi
合成的反义寡脱氧核苷酸序列互补的信使RNA（mRNA）编码的人金属硫蛋白（MT）II的制备和测试其抑制组成型和镉诱导的MT蛋白合成的能力在神经母细胞瘤-IMR和Chang肝细胞培养。还制备了正义寡核苷酸并作为其序列特异性效应的对照进行测试。通过使用聚凝胺载体增强了寡核苷酸进入细胞，使得10 μ M剂量的寡核苷酸的近30%显示与细胞相关。MT蛋白合成的反义寡核苷酸抑制使两种细胞类型对镉毒性更敏感。然而，正义寡核苷酸对MT蛋白合成或对镉毒性的敏感性没有影响。
A synthetic antisense oligodeoxyribonucleotide with sequence complementary to the messenger RNA (mRNA) coding for human metallothionein (MT) II was prepared and tested for its ability to inhibit both constitutive- and cadmium-induced MT protein synthesis in neuroblastoma-IMR and Chang liver cells in culture. The sense oligonucleotide was also prepared and tested as a control for its sequence-specific effects. Oligonucleotide entry into cells was enhanced through the use of a polybrene carrier so that nearly 30% of a 10 microM dose of oligonucleotide was shown to be associated with cells. The antisense oligonucleotide inhibition of MT protein synthesis rendered both cell types more sensitive to cadmium toxicity. However, the sense oligonucleotide had no effects on either MT protein synthesis or sensitivity to cadmium toxicity.