In Planta Biocontrol of Pectobacterium atrosepticum by Rhodococcus erythropolis Involves Silencing of Pathogen Communication by the Rhodococcal Gamma-Lactone Catabolic Pathway.
In Planta Biocontrol of Pectobacterium atrosepticum by Rhodococcus erythropolis Involves Silencing of Pathogen Communication by the Rhodococcal Gamma-Lactone Catabolic Pathway.
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DOI：
10.1371/journal.pone.0066642
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Latour X
中科院分区：
文献类型：
--
作者：
Barbey C;Crépin A;Bergeau D;Ouchiha A;Mijouin L;Taupin L;Orange N;Feuilloley M;Dufour A;Burini JF;Latour X
许多革兰氏阴性菌的毒力是在基于N-酰基高丝氨酸内酯的合成和感知的群体感应过程的控制下。红串红球菌是一种革兰氏阳性细菌，最近被提出作为一种生物防治剂用于植物保护以对抗软腐病菌，包括果胶杆菌。在这里，我们表明，γ-内酯分解代谢途径的R。红串菌（erythropolis）破坏果胶杆菌属（Pectobacterium）通讯并防止植物软腐病。我们报告的第一个表征和演示的N-酰基高丝氨酸内酯淬灭在植物。特别是，我们描述了R的转录。红串菌内酯酶基因，编码该途径的关键酶，以及随后的内酯分解。该代谢途径在生防活性中的作用已通过从R.红串菌及其在大肠杆菌中的异源表达。γ-内酯分解代谢途径是由病原体传播而不是病原体入侵诱导的。因此，这是一种新的和不寻常的生物控制途径，不同于以前描述的保护植物免受植物病原体的影响。这些发现还表明存在有助于植物保护的额外途径。
The virulence of numerous Gram-negative bacteria is under the control of a quorum sensing process based on synthesis and perception of N-acyl homoserine lactones. Rhodococcus erythropolis, a Gram-positive bacterium, has recently been proposed as a biocontrol agent for plant protection against soft-rot bacteria, including Pectobacterium. Here, we show that the γ-lactone catabolic pathway of R. erythropolis disrupts Pectobacterium communication and prevents plant soft-rot. We report the first characterization and demonstration of N-acyl homoserine lactone quenching in planta. In particular, we describe the transcription of the R. erythropolis lactonase gene, encoding the key enzyme of this pathway, and the subsequent lactone breakdown. The role of this catabolic pathway in biocontrol activity was confirmed by deletion of the lactonase gene from R. erythropolis and also its heterologous expression in Escherichia coli. The γ-lactone catabolic pathway is induced by pathogen communication rather than by pathogen invasion. This is thus a novel and unusual biocontrol pathway, differing from those previously described as protecting plants from phytopathogens. These findings also suggest the existence of an additional pathway contributing to plant protection.