Transforming Growth Factor-β1 is Constitutively Secreted by Chinese Hamster Ovary Cells and is Functional in Human Cells
Transforming Growth Factor-β1 is Constitutively Secreted by Chinese Hamster Ovary Cells and is Functional in Human Cells
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DOI：
10.1002/bit.23217
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发表时间：
2011-11-01
影响因子：
3.8
通讯作者：
Burchell, Joy M.
中科院分区：
文献类型：
--
作者：
Beatson, Richard;Sproviero, Daisy;Burchell, Joy M.
中国仓鼠卵巢(CHO)细胞被广泛用于生产临床和学术研究用的重组蛋白。在细菌不能使用的情况下，它们对于糖蛋白的生产尤其重要。转化生长因子β1是一种跨物种高度保守的细胞因子，具有多种免疫和非免疫效应。我们发现CHOK1，制药业最常用的CHO克隆，结构性地分泌潜在的转化生长因子β1，这种仓鼠转化生长因子β1对人类细胞具有活性，诱导深刻的免疫效应。据我们所知，CHOK1细胞产生转化生长因子β1尚未见文献报道。由于转化生长因子β1对不同类型的细胞具有强大的多效性作用，以及CHO细胞被用于生产大量的临床和非临床产品，我们的发现与依赖重组蛋白的研究高度相关。生物技术。比昂斯。2011；108：2759-2764。(C)2011年威利期刊公司。
Chinese hamster ovary (CHO) cells are widely used for the production of recombinant proteins for clinical use as well as academic research. They are particularly important for the production of glycoproteins where bacteria cannot be used. TGF beta 1 is a potent cytokine highly conserved across species with multiple immunological and non-immunological effects. We have discovered that CHOK1, the CHO clone most commonly used by the pharmaceutical industry, constitutively secretes latent TGF beta 1 and that this hamster TGF beta 1 is active on human cells inducing profound immunological effects. As far as we are aware, the production of TGF beta 1 by CHOK1 cells has not been reported before in the literature. As TGF beta 1 exerts powerful and pleiotropic effects on diverse cell types, and as CHO cells are used to produce a large number of clinical and non-clinical products, our findings are highly relevant to studies that rely on recombinant proteins. Biotechnol. Bioeng. 2011; 108: 2759-2764. (C) 2011 Wiley Periodicals, Inc.