Predominance of the DEN-3 genotype during the recent dengue outbreak in Bangladesh.
Predominance of the DEN-3 genotype during the recent dengue outbreak in Bangladesh.
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孟加拉国最近爆发的登革热疫情期间 DEN-3 基因型占主导地位。
DOI：
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发表时间：
2002
影响因子：
0.2
通讯作者：
Maruf Hassan
中科院分区：
文献类型：
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作者：
Monowar Aziz;K. N. Hasan;M. A. Hasanat;Maliha Afshan Siddiqui;M. Salimullah;A. K. Chowdhury;Moslehuddin Ahmed;M. N. Alam;Maruf Hassan
孟加拉国最近爆发的登革热具有许多致命的并发症。由于不同的登革热病毒的临床毒力不同，因此开展了一项孟加拉国登革热分子基因分型的研究。用反转录-聚合酶链式反应确定病毒的基因分型，使用产生511个碱基产物的寡核苷酸通用引物。扩增产物经2%琼脂糖凝胶溴化乙锭染色后在紫外光透光仪上显色，扩增出482(DEN-1)、119(DEN-2)、290(DEN-3)和392(DEN-4)。在45名临床诊断的登革热患者(平均年龄28岁；男性/女性30/15)中，19人(42.2%)的血液中可检测到病毒RNA。然而，在30名患者发烧的前5天，这一频率为60%(18/30)，这意味着发烧后越早抽取血清，检测到病毒RNA的机会就越大。除2例DEN-2感染者外，其余患者均检出DEN-3。DEN-2(2例)和DEN-4(1例)与DEN-3合并感染。所有患者都表现为发热、食欲不振和呕吐；许多患者出现头痛和全身疼痛；少数患者出现皮疹。约四分之一的患者曾经历过出血，少数患者出现腹水、胸腔积液和中枢神经系统症状，在随后的采样中，病毒核糖核酸阳性的患者抗登革热免疫球蛋白M也呈阳性(p=0.007)。这项研究表明，在孟加拉国最近爆发的登革热期间，登革热-3感染占主导地位，偶尔与其他类型的登革热合并感染。
A recent outbreak of dengue in Bangladesh was marked by many fatal complications. As clinical virulence varies among the genotypes of dengue virus, a study was conducted to investigate the molecular genotypes of dengue in Bangladesh. Reverse transcription polymerase chain reaction was used to determine viral genotypes using oligonucleotide generic primers that produce a 511 bp product. The resulting product was typed by nested PCR with strain-specific primers, yielding 482 (DEN-1), 119 (DEN-2), 290 (DEN-3) and 392 (DEN-4), visualized on UV transilluminator after electrophoresis on 2% agarose gel stained with ethidium bromide. Of 45 clinically diagnosed dengue patients (mean age 28 years; male/female 30/15), 19 (42.2%) had detectable viral RNA in their blood. However, during the first 5 days of fever in 30 patients, the frequency was 60% (18/30), implying that the sooner serum is drawn after the fever, the greater the chances of detecting viral RNA. DEN-3 was detected in all except 2 patients who were infected with DEN-2. DEN-2 (two cases) and DEN-4 (one case) were present as co-infections with DEN-3. All of the patients presented with fever, anorexia and vomiting; many had headache and general body ache; a few had a rash. About a quarter had suffered episodes of bleeding, while ascites, pleural effusion and CNS symptoms were found in a few patients Patients positive for viral RNA were also positive for anti-dengue IgM (p=0.007) in subsequent sampling. The study suggests the predominance of DEN-3 infection with occasional co-infection with other types, during the recent outbreak of dengue in Bangladesh.