Extracts from benthic anatoxin-producing Phormidium are toxic to 3 macroinvertebrate taxa at environmentally relevant concentrations
Extracts from benthic anatoxin-producing Phormidium are toxic to 3 macroinvertebrate taxa at environmentally relevant concentrations
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DOI：
10.1002/etc.4243
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发表时间：
2018-11-01
影响因子：
4.1
通讯作者：
Wood, Susanna A.
中科院分区：
文献类型：
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作者：
Anderson, Brian;Voorhees, Jennifer;Wood, Susanna A.
产毒素蓝藻在全球河流和溪流中不断增加，导致人们越来越担心它们对水生生态系统的潜在影响。本研究的目的是培养现场收集的席藻在实验室中，确定个别物种，进行化学分析，以确定氰毒素，并进行毒性试验，以调查这种属的潜在影响流健康。淡水毒性测试用标准的美国环境保护署无脊椎动物测试方案进行，其中培养水用于生长从俄罗斯河（CA，USA）分离的3种席藻菌株。酶联免疫吸附试验用于测量总类毒素浓度。3株席藻的培养沃茨对可疑网纹蚤、透明蚤和稀释摇蚊均具有较高的毒性。梭对席藻1、2和3株的7天存活半致死浓度分别为0.71、0.49和0.56g/L。7-d的25%生长抑制浓度分别为0.55、0.32和0.30g/L。稀释摇蚊的存活率在浓度
Toxin-producing cyanobacteria are increasing in rivers and streams globally, leading to growing concerns over their potential impacts on aquatic ecosystems. The present study was designed to culture field-collected Phormidium in the laboratory, identify individual species, conduct chemical analyses to identify cyanotoxins, and conduct toxicity tests to investigate the potential for this genera to impact stream health. Freshwater toxicity tests were conducted with standard US Environmental Protection Agency invertebrate test protocols with culture water used to grow 3 Phormidium strains isolated from the Russian River (CA, USA). Enzyme linked immunosorbent assays were used to measure total anatoxin concentrations. Culture waters from the 3 Phormidium strains were highly toxic to Ceriodaphnia dubia, Hyalella azteca, and Chironomus dilutus. The C. dubia 7-d survival median lethal concentrations were 0.71, 0.49, and 0.56g/L anatoxin for Phormidum strains 1, 2, and 3, respectively. The 7-d reproduction inhibitory concentrations, 25% were 0.55, 0.32, and 0.30g/L anatoxin for strains 1, 2, and 3, respectively. Chironomus dilutus survival was reduced at concentrations