Correlation of OGR1 with proliferation and apoptosis of breast cancer cells
Correlation of OGR1 with proliferation and apoptosis of breast cancer cells
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DOI：
10.3892/ol.2019.10121
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发表时间：
2019-03
期刊：
影响因子：
2.9
通讯作者：
Jianguo Zhang;Lei Che;Wenkai Sun;Jian Shang;M. Hao;Mengzi Tian
中科院分区：
文献类型：
--
作者：
Jianguo Zhang;Lei Che;Wenkai Sun;Jian Shang;M. Hao;Mengzi Tian
探讨卵巢癌G蛋白偶联受体1（OGR 1）蛋白对乳腺癌细胞增殖和凋亡的影响及其分子机制。利用乳腺癌细胞瞬时转染OGR 1真核表达载体，构建了高表达OGR 1的MCF-7细胞株。同时，用空载体转染细胞作为对照。研究了高表达OGR 1对细胞生长、增殖、凋亡等能力的影响。此外，研究了高表达OGR 1对丝氨酸-苏氨酸激酶（AKT）、p53等基因的影响。凋亡实验证明，乳腺癌细胞中高表达OGR 1蛋白能有效增加细胞凋亡比例。细胞增殖实验显示，与OGR 1低表达的乳腺癌细胞相比，OGR 1高表达的乳腺癌细胞的生长和增殖能力受到一定程度的抑制。Western blotting结果显示，OGR 1高表达的乳腺癌细胞中p53基因和蛋白表达水平均升高。OGR 1低表达与高表达乳腺癌细胞AKT蛋白表达无显著性差异。但OGR 1高表达的乳腺癌细胞中磷酸化AKT（p-AKT）蛋白含量低于OGR 1低表达的乳腺癌细胞。乳腺癌细胞的增殖和凋亡受OGR 1表达变化的影响，并与AKT和p53基因表达水平有一定的相关性，但详细的分子机制还需进一步研究。
Effects of ovarian cancer G-protein-coupled receptor 1 (OGR1) protein on proliferation and apoptosis of breast cancer cells, as well as its molecular mechanism were investigated. The MCF-7 cell line highly expressed OGR1 was constructed by transient transfection of eukaryotic expression vector using breast cancer cells. At the same time, cells were transfected with empty vector as controls. The effects of highly expressed OGR1 on cell growth, proliferation, apoptosis and other abilities were identified. In addition, the effects of highly expressed OGR1 on serine-threonine kinase (AKT), p53 and other genes were studied. It was proved in apoptosis experiment that highly expressed OGR1 protein in breast cancer cells could effectively increase the proportion of apoptosis of cells. Cell proliferation experiment revealed that the growth and proliferation abilities of breast cancer cells with highly expressed OGR1 were inhibited to some extent, compared with those of breast cancer cells with low expression of OGR1. Results of western blotting showed that the gene and protein expression levels of p53 in breast cancer cells with highly expressed OGR1 were increased. There was no significant difference in protein expression of AKT between breast cancer cells with low expression of OGR1 and those with highly expressed OGR1. However, the protein content of phosphorylated-AKT (p-AKT) in breast cancer cells with highly expressed OGR1 was lower than that in breast cancer cells with low expression of OGR1. The proliferation and apoptosis of breast cancer cells are influenced by the changes of OGR1 expression, which are correlated with the gene expression levels of AKT and p53 to some extent, but the detailed molecular mechanism requires additional study.