Strain Specificity and Simultaneous Transmission of Closely Related Strains of a Potyvirus by Myzus persicae
Strain Specificity and Simultaneous Transmission of Closely Related Strains of a Potyvirus by Myzus persicae
复制标题
桃蝇马铃薯Y病毒属密切相关株的株系特异性及同时传播
DOI：
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发表时间：
2012
影响因子：
2.2
通讯作者：
J. Whitworth
中科院分区：
文献类型：
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作者：
R. Srinivasan;Darren G. Hall;Felix A Cervantes;J. M. Álvarez;J. Whitworth
摘要
马铃薯 Y 病毒 (PVY) 是马铃薯病毒属的一种，由蚜虫以非持久性方式传播。 PVY 严重影响全球马铃薯生产。 PVY菌株的单一和混合感染，即PVYO、PVYNTN和PVYN:O在马铃薯系统中很常见。然而，关于蚜虫同时传播多种 PVY 菌株的能力、与同时传播相关的特异性以及影响特异性的因素的可用信息有限。进行了蚜虫介导的传播实验，以测试单个蚜虫使用 PVY 指示宿主传播多种菌株的能力。初步结果显示，蚜虫可以同时传播至少两种病毒株。随后，使用马铃薯植物测试了蚜虫介导的三种双菌株组合的传播。对于所有三种双毒株组合，单个蚜虫同时传播两种病毒株。在涉及PVYNTN的所有蚜虫介导的双菌株感染中，PVYNTN感染率大于第二菌株和双菌株组合的感染率，表明与PVY菌株传播相关的特异性。将蚜虫介导的传播实验的结果与通过机械传播获得的结果进行比较。一般来说，蚜虫介导的PVY感染率低于机械传播的感染率。与蚜虫介导的传播不同，双菌株接种中的成分菌株在机械传播过程中并未消除。这些结果表明，也可能存在与蚜虫介导的密切相关的 PVY 菌株传播相关的干扰。也许，观察到的特异性和/或干扰可以解释近年来PVYNTN和其他坏死菌株发病率的增加。
ABSTRACT
Potato virus Y (PVY), a Potyvirus, is transmitted by aphids in a nonpersistent manner. PVY severely affects potato production worldwide. Single and mixed infections of PVY strains, namely PVYO, PVYNTN, and PVYN:O are a common occurrence in potato systems. However, information available on the ability of aphids to simultaneously transmit multiple PVY strains, specificity associated with simultaneous transmission, and factors affecting specificity are limited. Aphid-mediated transmission experiments were conducted to test the ability of individual aphids to transmit multiple strains using a PVY indicator host. Preliminary results revealed that aphids can transmit at least two viral strains simultaneously. Subsequently, aphid-mediated transmission of three dual-strain combinations was tested using potato plants. Individual aphids transmitted two viral strains simultaneously for all three dual-strain combinations. In all aphid-mediated dual-strain infections involving PVYNTN, the rate of PVYNTN infection was greater than the infection rates of the second strain and dual-strain combinations, indicating specificity associated with transmission of PVY strains. Results of aphidmediated transmission experiments were compared with results obtained through mechanical transmission. In general, PVY infection rates from aphid-mediated transmission were lower than the rates obtained through mechanical transmission. Unlike aphid-mediated transmission, component strains in dual-strain inoculations were not eliminated during mechanical transmission. These results suggest that there may also be interference associated with aphid-mediated transmission of closely related PVY strains. Perhaps, the observed specificity and / or interference may explain the increase in the incidence of PVYNTN and other necrotic strains in recent years.