ELECTRON SPIN RESONANCE STUDIES ON MELANIN
ELECTRON SPIN RESONANCE STUDIES ON MELANIN
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DOI：
10.1016/s0006-3495(64)86797-7
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发表时间：
1964-01-01
影响因子：
3.4
通讯作者：
ZAHLAN, AB
中科院分区：
文献类型：
--
作者：
BLOIS, MS;MALING, JE;ZAHLAN, AB
电子自旋共振(e.s.r.)在500°K到4.2°K的温度范围内对鱿鱼黑色素进行了观察，并对黑色素进行了各种化学处理对E.S.R.的影响。对光谱进行了研究。研究结果表明，该黑色素的顺磁性在500°K到4.2°K之间遵循居里定律，自旋信号可以通过向黑色素中添加铜离子来消除，光学和电子自旋共振。黑色素的吸收是独立的，因为任何一种都可以减少或消除，而不会影响另一种。对合成黑色素进行了类似的研究，这些合成黑色素是通过自氧化或通过一些联苯酚的酶氧化产生的。结果发现，E.S.R.这些合成黑色素的信号与鱿鱼黑色素的信号惊人地相似(在线宽、线形和g值方面)。结论是，观察到的未配对电子与黑色素聚合物中捕获的自由基有关，黑色素的生物合成可能涉及自由基机制，这些物理数据符合Nicolaus的概念，即黑色素是一种高度不规则的三维聚合物。
Electron spin resonance (e.s.r.) observations of squid melanin have been conducted over the temperature range 500°K to 4.2°K, and the effect of various chemical treatments of the melanin upon the e.s.r. spectrum has been studied. The findings have shown that the paramagnetism of this melanin follows the Curie Law from 500°K to 4.2°K, that the spin signal can be eliminated by the addition of Cu++to the melanin, and that the optical and e.s.r. absorptions of melanin are independent since either can be reduced or eliminated without affecting the other. Similar studies on synthetic melanins produced by autoxidation or by enzymatic oxidation of a number of biphenols were carried out. It was found that the e.s.r. signals of these synthetic melanins were strikingly similar (with respect to line width, line shape, andg-value) with those of squid melanin. It is concluded that the unpaired electrons observed are associated with trapped free radicals in the melanin polymer, that the biosynthesis of melanin may involve a free radical mechanism, and that these physical data are in accord with the concept of Nicolaus that melanin is a highly irregular, three-dimensional, polymer.